HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Personal Folders Download to citation manager Permission Requests Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Bolling, S. F. Articles by Childs, K. F. Search for Related Content PubMed PubMed Citation Articles by Bolling, S. F. Articles by Childs, K. F.

The Journal of Thoracic and Cardiovascular Surgery, Vol 103, 73-77, Copyright © 1992 by The American Association for Thoracic Surgery and The Western Thoracic Surgical Association

ARTICLES

Enhanced myocardial protection during global ischemia with 5'- nucleotidase inhibitors

SF Bolling, DA Olszanski, EL Bove and KF Childs
Thoracic Surgery Research Laboratory, University of Michigan Medical School, Ann Arbor.

Depletion of adenosine triphosphate precursors, such as myocardial adenosine, during global ischemia results in poor postischemic adenosine triphosphate repletion and functional recovery. Neonatal hearts may be more resistant to this deleterious effect of ischemia, because they are characterized by low 5'-nucleotidase activity, which may result in higher sustained endogenous myocardial adenosine triphosphate precursor levels during ischemia. Adult hearts, however, have high levels of 5'-nucleotidase activity leading to depleted precursors during ischemia and poor postischemic functional recovery. Augmenting myocardial adenosine exogenously during ischemia in adult hearts has a beneficial effect on recovery. The present study tested if preservation of nucleotide precursors, better adenosine triphosphate repletion, and enhanced postischemic myocardial recovery in adult hearts could be achieved with a "neonatal" strategy. Therefore 5'- nucleotidase inhibitors were administered to isolated, perfused adult rabbit hearts subjected to 120 minutes of ischemia (at 34 degrees C) to determine if this improved functional recovery. Hearts received St. Thomas' Hospital cardioplegic solution (control hearts) or cardioplegic solution containing 5'-nucleotidase inhibitors: pentoxifylline, thioinosine, [s-(p-nitrophenyl)-4-thioinosine], or thioinosine's dimethyl sulfoxide vehicle alone. After ischemia and reperfusion, recovery of systolic function, diastolic function, and myocardial oxygen consumption was significantly better with 5'-nucleotidase inhibition. No changes in coronary flow were noted. We speculate and are pursuing the theory that the mechanism of 5'-nucleotidase inhibition's favorable action is due to preventing the catabolism, transport, and loss of nucleotide precursors during ischemia, maintaining adenosine triphosphate precursor availability.

This article has been cited by other articles:

				T. Doenst, C. Schlensak, and F. Beyersdorf Cardioplegia in pediatric cardiac surgery: do we believe in magic? Ann. Thorac. Surg., May 1, 2003; 75(5): 1668 - 1677. [Abstract] [Full Text] [PDF]

				Y. Xia, G. Khatchikian, and J. L. Zweier Adenosine Deaminase Inhibition Prevents Free Radical-mediated Injury in the Postischemic Heart J. Biol. Chem., April 26, 1996; 271(17): 10096 - 10102. [Abstract] [Full Text] [PDF]

				A. K. Pridjian, E. L. Bove, S. F. Bolling, K. F. Childs, K. M. Brosamer, and F. M. Lupinetti Developmental differences in myocardial protection in response to 5'-nucleotidase inhibition J. Thorac. Cardiovasc. Surg., February 1, 1994; 107(2): 520 - 526. [Abstract] [Full Text]