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The Journal of Thoracic and Cardiovascular Surgery, Vol 106, 912-917, Copyright © 1993 by The American Association for Thoracic Surgery and The Western Thoracic Surgical Association
FM Lupinetti, TT Tsai, JM Kneebone and EL Bove
Cryopreserved human allograft valves are useful in a variety of cardiac
operations. The presence or absence of endothelial cells on allografts may
be important in determining immunogenicity and ultimate graft longevity.
The purpose of this study was to determine whether endothelial cells are
present on cryopreserved human allografts. Portions of cryopreserved
allografts (35 valve leaflets, 96 pieces of arterial wall) not used at
operation were studied. For comparison, untreated tissues (44 valve
leaflets, 46 pieces of arterial wall) were obtained from structurally
normal hearts and lungs removed or inserted at the time of transplantation
and from pathologic tissues obtained during operations for congenital heart
defects. A monolayer of cells from the luminal surface of each specimen was
harvested by means of a Hautchen preparation. The monolayer was stained
with fluorescein isothiocyanate-labeled Ulex europaeus I, a lectin with
strong affinity for human endothelium. Positive staining with fluorescein
was considered to be evidence for the presence of human endothelium.
Endothelial cells were observed on 21 of 131 (16%) cryopreserved allograft
specimens and on 70 of 90 (78%) untreated tissues (p < 0.001). These
results show that cryopreservation typically results in the loss of
endothelium from aortic and pulmonary valve allografts. These findings may
have important implications for the immunologic response of the host to
allograft implantation.
ARTICLES
Effect of cryopreservation on the presence of endothelial cells on human valve allografts
Department of Surgery, University of Michigan School of Medicine, Ann Arbor.
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R. N. Mitchell, R. A. Jonas, and F. J. Schoen Pathology Of Explanted Cryopreserved Allograft Heart Valves: Comparison With Aortic Valves From Orthotopic Heart Transplants J. Thorac. Cardiovasc. Surg., January 1, 1998; 115(1): 118 - 127. [Abstract] [Full Text] [PDF]
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K. Tamura, M. Jones, I. Yamada, and V. J. Ferrans A comparison of failure modes of glutaraldehyde-treated versus antibiotic-preserved mitral valve allografts implanted in sheep J. Thorac. Cardiovasc. Surg., July 1, 1995; 110(1): 224 - 238. [Abstract] [Full Text]
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M. G. Hazekamp, D. R. Koolbergen, J. Braun, H. Sugihara, C. J. Cornelisse, Y. A. Goffin, and H. A. Huysmans In situ hybridization: A new technique to determine the origin of fibroblasts in cryopreserved aortic homograft valve explants J. Thorac. Cardiovasc. Surg., July 1, 1995; 110(1): 248 - 257. [Abstract] [Full Text]
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F. M. Lupinetti and J. M. Kneebone Cryopreserved heart valve allografts can have a normal endothelium J. Thorac. Cardiovasc. Surg., November 1, 1994; 108(5): 987 - 988. [Full Text]
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D. C. McGiffin Invited letter concerning: Leaflet viability and the durability of the allograft aortic valve J. Thorac. Cardiovasc. Surg., November 1, 1994; 108(5): 988 - 990. [Full Text]
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