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J Thorac Cardiovasc Surg 1994;107:717-723
© 1994 Mosby, Inc.

SURGERY FOR ACQUIRED HEART DISEASE

Effects of preservation techniques on in vivo expression of adhesion molecules by aortic valve allografts

Ann Arbor, Mich.

Supported by grant R01 HL 42426 from the National Heart, Lung, and Blood Institute.

Received for publication Jan. 21, 1993. Accepted for publication June 29, 1993. Address for reprints: Flavian M. Lupinetti, MD, Children's Hospital and Medical Center, 4800 Sand Point Way, Seattle, WA 98105.

Abstract

Methods of sterilization and preservation of aortic valve allografts influence graft longevity. The effect of storage techniques on valve durability may be mediated by alterations in the immunologic properties of the allograft, which are reflected by expression of leukocyte adhesion molecules. Rat aortic valve grafts were transplanted in the fresh state, after cryopreservation (-196° C), or after storage at 4° C for 1 to 21 days. Syngeneic and strongly allogeneic valves were transplanted for 4 hours to 21 days and were retrieved for immunohistochemical staining for expression of leukocyte adhesion molecules. Unimplanted valves and transplanted syngeneic valves, regardless of storage methods, exhibited little or no expression of leukocyte adhesion molecules. Fresh allogeneic valves expressed all molecules, indicating up-regulation, at all time intervals studied. Cryopreserved allogeneic valves demonstrated no leukocyte adhesion molecules at 4 hours or 2 days and weak reactivity at 10 and 21 days. Allogeneic valves stored at 4° C, regardless of the duration of storage, demonstrated weak expression of all molecules at 10 days and strong expression at 21 days. Expression of leukocyte adhesion molecules requires an allogeneic environment and may precede immune-mediated injury. Reduced expression of leukocyte adhesion molecules resulting from storage may predict a diminished immunologic response. Cryopreservation (-196° C) causes the greatest delay and diminution of expression of leukocyte adhesion molecules. (J THORACCARDIOVASCSURG1994;107:717-23)

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