HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Personal Folders Download to citation manager Permission Requests Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Zhang, J. C. Articles by Binder, B. R. Search for Related Content PubMed PubMed Citation Articles by Zhang, J. C. Articles by Binder, B. R.

J Thorac Cardiovasc Surg 1995;109:1059-1065
© 1995 Mosby, Inc.

SURGERY FOR ACQUIRED HEART DISEASE

Growth and fibrinolytic parameters of human umbilical vein endothelial cells seeded onto cardiovascular grafts

Vienna, Austria, and Beijing, People's Republic of China

This work was supported by a North-South Dialogue scholarship from The Austrian Academic Exchange Service (ÖAD) to J.C.Z. and by grants from The Austrian Fund for the Promotion of Scientific Research to J.W. (P9479) and B.R.B. (P8011 and P8854).

Received for publication June 14, 1994. Accepted for publication Oct. 25, 1994. Address for reprints: Johann Wojta, PhD, Department of Medical Physiology, Lab. Clin. Exp. Physiol., University of Vienna, Schwarzspanierstraße 17, A-1090 Vienna, Austria.

Abstract

It was the aim of this study to investigate possible effects of biomaterials used to produce vascular grafts on the fibrinolytic system of endothelial cells. Therefore growth conditions for human umbilical vein endothelial cells on polytetrafluoroethylene and on polyurethane were optimized. Tissue culture polystyrene was used as a control material. We could demonstrate that precoating of the materials with fibronectin significantly increased the growth rate of human umbilical vein endothelial cells on these materials. Furthermore, we showed that human umbilical vein endothelial cells grown on polytetrafluoroethylene or polyurethane released more plasminogen activator inhibitor-1 and tissue type-plasminogen activator into the conditioned media than did human umbilical vein endothelial cells grown on tissue culture polystyrene. Human umbilical vein endothelial cells cultured on polytetrafluoroethylene also deposited more plasminogen activator inhibitor-1 into the extracellular matrix than did control cells grown on tissue culture polystyrene. Our results give evidence that human umbilical vein endothelial cells grown on two biomaterials used to construct vascular grafts, namely polytetrafluoroethylene and polyurethane, produce tissue-type plasminogen activator as well as plasminogen activator inhibitor-1, two major components of the fibrinolytic system also expressed by endothelial cells in vivo. In conclusion, our data suggest that endothelial cells grown on vascular grafts show functional integrity concerning their fibrinolytic system, which in turn might contribute to reduce the thrombogenic properties of the graft material. (J THORAC CARDIOVASC SURG 1995;109:1059-65)

This article has been cited by other articles:

				G. H.L. Tang, S. Fazel, R. D. Weisel, G. S. Van Arsdell, and R.-K. Li Cardiovascular Tissue Engineering Therapy: So Near, So Far? Ann. Thorac. Surg., June 1, 2005; 79(6): 1831 - 1833. [Full Text] [PDF]

				H. M. Nugent and E. R. Edelman Tissue Engineering Therapy for Cardiovascular Disease Circ. Res., May 30, 2003; 92(10): 1068 - 1078. [Abstract] [Full Text] [PDF]