HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Personal Folders Download to citation manager Permission Requests Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Yokomise, H. Articles by Hitomi, S. Search for Related Content PubMed PubMed Citation Articles by Yokomise, H. Articles by Hitomi, S.

J Thorac Cardiovasc Surg 1995;110:382-385
© 1995 Mosby, Inc.

GENERAL THORACIC SURGERY

RELIABLE CRYOPRESERVATION OF TRACHEA FOR ONE MONTH IN A NEW TREHALOSE SOLUTION

Kyoto, Japan

From the Division of Thoracic Surgery, Chest Disease Research Institute, Kyoto University, Kyoto, Japan.

Received for publication July 20, 1994. Accepted for publication Dec. 2, 1994. Address for reprints: H. Yokomise, MD, Division of Thoracic Surgery, Chest Disease Research Institute, Kyoto University, 53 Shogoin-Kawaharacho, Sakyo-ku, Kyoto, 606, Japan.

Abstract

We previously reported that trehalose, a reduced disaccharide, was effective in the preservation of lungs. In this study, we investigated the possibility of prolonged cryopreservation of tracheas in a preservative solution containing trehalose. Five rings of cervical trachea were removed and immersed in the preservative solution. The harvested tracheas were then cryopreserved and stored in a deep freezer at -85° C. One month later, five rings of mediastinal trachea were removed. The cryopreserved cervical tracheas were thawed and autotransplanted in place of the excised mediastinal trachea (n = 6). The anastomotic site and graft were then covered with an omental pedicle. All six animals survived for more than 6 months. All grafts survived without any evidence of atrophy or stenosis. Microscopic examination of the grafts showed that the integrity of the tracheal tissues was maintained. Our findings show that consistent cryopreservation of the trachea for 1 month is possible in a preservative solution containing trehalose. (J THORACCARDIOVASCSURG1995;110:382-5)

This article has been cited by other articles:

				M. Kawamura, Y. Izumi, N. Tsukada, K. Asakura, H. Sugiura, H. Yashiro, K. Nakano, S. Nakatsuka, S. Kuribayashi, and K. Kobayashi Percutaneous cryoablation of small pulmonary malignant tumors under computed tomographic guidance with local anesthesia for nonsurgical candidates J. Thorac. Cardiovasc. Surg., May 1, 2006; 131(5): 1007 - 1013. [Abstract] [Full Text] [PDF]

				T. Okamoto, Y. Yamamoto, M. Gotoh, C.-L. Huang, T. Nakamura, Y. Shimizu, Y. Tabata, and H. Yokomise Slow release of bone morphogenetic protein 2 from a gelatin sponge to promote regeneration of tracheal cartilage in a canine model J. Thorac. Cardiovasc. Surg., February 1, 2004; 127(2): 329 - 334. [Abstract] [Full Text] [PDF]

				K. Kushibe, K. Nezu, K. Nishizaki, M. Takahama, and S. Taniguchi Tracheal allotransplantation maintaining cartilage viability with long-term cryopreserved allografts Ann. Thorac. Surg., May 1, 2001; 71(5): 1666 - 1669. [Abstract] [Full Text] [PDF]

				M. Hashimoto, R. Nakanishi, M. Umesue, H. Muranaka, M. Hachida, and K. Yasumoto Feasibility of cryopreserved tracheal xenotransplants with the use of short-course immunosuppression J. Thorac. Cardiovasc. Surg., February 1, 2001; 121(2): 0241 - 248. [Abstract] [Full Text] [PDF]

				T. Aoki, Y. Yamato, M. Tsuchida, T. Souma, K. Yoshiya, T. Watanabe, and J.-i. Hayashi Successful tracheal transplantation using cryopreserved allografts in a rat model Eur. J. Cardiothorac. Surg., August 1, 1999; 16(2): 169 - 173. [Abstract] [Full Text] [PDF]

				R. Nakanishi, M. Hashimoto, H. Muranaka, M. Umesue, H. Kohno, and K. Yasumoto MAXIMAL PERIOD OF CRYOPRESERVATION WITH THE BICELL BIOFREEZING VESSEL FOR RAT TRACHEAL ISOGRAFTS J. Thorac. Cardiovasc. Surg., June 1, 1999; 117(6): 1070 - 1076. [Abstract] [Full Text] [PDF]

				T. Mukaida, N. Shimizu, M. Aoe, A. Andou, H. Date, K. Okabe, M. Yamashita, and S. Ichiba Experimental study of tracheal allotransplantation with cryopreserved grafts J. Thorac. Cardiovasc. Surg., August 1, 1998; 116(2): 262 - 266. [Abstract] [Full Text] [PDF]

				K. Inutsuka, K. Kawahara, T. Takachi, K. Okabayashi, T. Shiraishi, and T. Shirakusa Reconstruction of Trachea and Carina With Immediate or Cryopreserved Allografts in Dogs Ann. Thorac. Surg., November 1, 1996; 62(5): 1480 - 1484. [Abstract] [Full Text]

				H. Yokomise, K. Inui, H. Wada, M. Ueda, S. Hitomi, and H. Itoh SPLIT TRANSPLANTATION OF THE TRACHEA: A NEW OPERATIVE PROCEDURE FOR EXTENDED TRACHEAL RESECTION J. Thorac. Cardiovasc. Surg., August 1, 1996; 112(2): 314 - 318. [Abstract] [Full Text]

				H. Yokomise, K. Inui, H. Wada, M. Ueda, and S. Hitomi LONG-TERM CRYOPRESERVATION CAN PREVENT REJECTION OF CANINE TRACHEAL ALLOGRAFTS WITH PRESERVATION OF GRAFT VIABILITY J. Thorac. Cardiovasc. Surg., May 1, 1996; 111(5): 930 - 934. [Abstract] [Full Text]