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J Thorac Cardiovasc Surg 1996;111:323-333
© 1996 Mosby, Inc.

GENERAL THORACIC SURGERY

MUTATIONS OF p53 IN BARRETT'S ESOPHAGUS AND BARRETT'S CANCER: A PROSPECTIVE STUDY OF NINETY-EIGHT CASES

Munich, Germany; London, Ontario, Canada; Tucson, Ariz.; Houston, Tex.

Supported by grants from the Deutsche Krebsgesellschaft (German Cancer Society)/Dr. Mildred Sheol Stiftung (M91/91/H81) to P. M. S. and A. H. H., the National Cancer Institute RO1 CA45187 to J. A. R., by National Cancer Institute Training Grant CA09611 to J. A. R., by gifts to the Division of Surgery from Tenneco and Exxon for the Core Laboratory Facility, and by the M. D. Anderson Cancer Center Support Core Grant (CA16672), by a grant from the Mathers Foundation to J. A. R., J. P. Bickell Foundation, Ontario Thoracic Society to A. G. C., Victoria Hospital Research Development Fund and the Department of Surgery Academic Enrichment Fund to A. G. C.

Address for reprints: J. A. Roth, MD, Department of Thoracic and Cardiovascular Surgery, The University of Texas M. D. Anderson Cancer Center, 1515 Holcombe Blvd., Box 109, Houston, TX 77030.

Abstract

We had previously identified p53 mutations in Barrett's esophagus and therefore began a multiinstitutional study to determine their significance as a marker for malignancy. Ninety-eight patients from four institutions were studied. Forty-eight patients (37 men and 11 women, mean age 56.2 years) had Barrett's esophagus with metaplasia or dysplasia but no evidence of malignancy at a mean follow-up of 2.2 years. Barrett's esophagus was classified as metaplasia with no evidence of dysplasia in 32 patients, as low-grade dysplasia in 13, and as high-grade dysplasia in three. The other 50 patients (46 men and four women, mean age 60.2 years) had adenocarcinoma arising in Barrett's esophagus. Tissues from normal stomach or esophagus, tumor, and Barrett's esophagus were obtained for deoxyribonucleic acid analysis by endoscopic biopsy from patients with Barrett's esophagus or cancer or during operations on some patients with Barrett's cancer. Exons 5 through 9 of the p53 gene were studied for mutations by single-strand conformational polymorphism analysis after polymerase chain reaction amplification. Mutations detected by single-strand conformational polymorphism analysis were confirmed by deoxyribonucleic acid sequencing. None of the tissue samples from patients with Barrett's esophagus alone and no dysplasia or low-grade dysplasia had any p53 mutations, but one of the three patients with high-grade dysplasia and no evidence of invasive malignancy did have a p53 mutation. Of the 50 patients with Barrett's cancer, however, 23 (46%) had p53 mutations in Barrett's epithelium, tumors, or both. Twenty of these patients had p53 mutations in the tumor only (n = 16) or in both tumor and Barrett's epithelium (n = 4), suggesting that the mutation plays a direct role in carcinogenesis. Mutations in Barrett's epithelium were found in one patient in the group without malignancy and in seven patients with cancer (one with no dysplasia, two with low-grade dysplasia, and five with high-grade dysplasia). In three patients with cancer, mutations occurred only in Barrett's epithelium, suggesting that such mutations may also be a marker for genomic instability. Mutations were predominantly found in exons 5, 7, and 8, and transitions from guanine to adenine were the most frequent changes. Mutations of p53 are clearly involved in the pathogenesis of Barrett's cancer for a subset of patients (46%), and the fact that we could detect mutations in premalignant Barrett's epithelium supports the hypothesis that p53 mutations may be a useful marker for patients at increased risk for development of invasive cancer. (J THORACCARDIOVASCSURG1996;111:323-33)

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