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J Thorac Cardiovasc Surg 1996;112:1002-1009
© 1996 Mosby, Inc.

CARDIAC AND PULMONARY REPLACEMENT

THE INFLUENCE OF CONCORDANT XENOGRAFTS ON THE HUMORAL AND CELL-MEDIATED IMMUNE RESPONSES TO SUBSEQUENT ALLOGRAFTS IN PRIMATES

Supported in part by National Institutes of Health grant PPG PO1HL36581-01A1.

Received for publication June 26, 1995 Revisions requested Nov. 29, 1995; revisions received Dec. 22, 1995 Accepted for publication Jan. 9, 1996. Address for reprints: Robert E. Michler, MD, Director, Cardiac Transplant Service, Director, Cardiac Transplant Research Laboratory, Columbia-Presbyterian Medical Center, Milstein Hospital Building, 7-435, 177 Fort Washington Ave, New York, NY 10032.

Abstract

The humoral and cell-mediated immune responses to subsequent allografts were determined in primate recipients after concordant xenotransplantation as a bridge to allotransplantation. Heterotopic heart transplants (n = 4) were performed from cynomolgus monkeys into ABH type–matched olive baboons followed 2 weeks later by allotransplantation from ABH type–matched baboon donors. Allografts were explanted at 8 weeks. All recipients underwent splenectomy at the time of xenotransplantation and received immunosuppression with cyclosporine, azathioprine, and methylprednisolone. Concordant xenotransplantation in these primates did not induce humoral or cell-mediated immune responses that jeopardized subsequent allografts. The degree of xenospecific immune reactivity, as determined by specific cytotoxicity of recipient T-cell lines derived from the xenograft and extent of histologic xenograft rejection, did not predict the severity of subsequent allograft rejection. In two of the four recipients, xenotransplantation induced an alloreactive humoral response against antigens expressed by the B cells of more than 50% of members from a panel of 12 unrelated baboons. In all recipients, priming with xenogeneic splenocytes in vitro induced an accelerated proliferative T-cell response to allogeneic lymphocytes from 16% of this panel. This study affirms the role of concordant xenografts as appropriate biologic bridges to human allotransplantation. However, our results suggest that xenoreactive baboon memory CD4 T cells may recognize major histocompatibility complex class II–like structures shared between the xenogeneic and allogeneic targets. The potential allorecognition induced by a xenograft may affect the process of subsequent allograft donor selection. (J THORAC CARDIOVASC SURG 1996;112:1002-9)

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