HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Personal Folders Download to citation manager Author home page(s): Satoshi Saito Noboru Motomura Edward A. Lefrak Permission Requests Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Saito, S. Articles by Lefrak, E. A. Search for Related Content PubMed PubMed Citation Articles by Saito, S. Articles by Lefrak, E. A.

J Thorac Cardiovasc Surg 1997;114:803-810
© 1997 Mosby, Inc.

CARDIAC AND PULMONARY REPLACEMENT

SPECIFIC EFFECTS OF ESTROGEN ON GROWTH FACTOR AND MAJOR HISTOCOMPATIBILITY COMPLEX CLASS II ANTIGEN EXPRESSION IN RAT AORTIC ALLOGRAFT

Supported by the Ernst Schering Research Foundation and National Institutes of Health grant R01HL58896.

Received for publication May 7, 1997 revisions requested June 30, 1997; revisions received July 30, 1997 accepted for publication July 30, 1997. Address for reprints: Marie L. Foegh, MD, DSc, Georgetown University Medical Center, 4000 Reservoir Rd., N.W., Building D, Room 397, Washington, DC 20007.

Abstract

Objective: Transplant arteriosclerosis is the major determinant for long-term survival of cardiac transplants. Estradiol treatment inhibits transplant arteriosclerosis. The objective of this study is to determine, in the absence of immunosuppression, the temporal effect of estradiol treatment on the expression of insulin-like growth factor, platelet-derived growth factor, basic fibroblast growth factor, and major histocompatibility complex class II antigen in rat aortic allografts. Methods: Orthotopic abdominal aortic allograft transplantation was performed in male rats with Brown-Norway rats used as donors and Lewis rats as recipients. The recipients (n = 50) were treated with estradiol 20 µg/kg per day or placebo by osmotic minipump for 2 days before the operation and until they were put to death on postoperative days 1, 3, 7, 14, or 21. The allografts were harvested and insulin-like growth factor, platelet-derived growth factor, basic fibroblast growth factor, and major histocompatibility complex class II antigen expression were determined by immunohistochemical staining. Myointimal thickening was measured by morphometric analysis. Results: In the placebo-treated group, insulin-like growth factor protein progressively increased in all three layers of the allograft, whereas platelet-derived growth factor protein peaked at day 3 and basic fibroblast growth factor protein increased only moderately. Estradiol treatment inhibited the continuous increase in insulin-like growth factor expression, the peak in platelet-derived growth factor expression at day 3, the moderate basic fibroblast growth factor increase at day 21, and major histocompatibility complex class II antigen expression in all three layers of the allograft at day 21. Intimal thickening of allografts from estradiol-treated recipients was twofold to threefold less than that of the placebo-treated recipients at day 21. Conclusion: The development of transplant arteriosclerosis is associated with an early alloimmune response involving sustained increase in insulin-like growth factor expression. Estradiol treatment of the recipient inhibits transplant arteriosclerosis and suppresses insulin-like growth factor and major histocompatibility complex class II antigen expression but not platelet-derived growth factor or basic fibroblast growth factor in all three layers of the allograft during the early posttransplantation alloimmune rejection phase.

This article has been cited by other articles:

				J. Adamski, Z. Ma, S. Nozell, and E. N. Benveniste 17{beta}-Estradiol Inhibits Class II Major Histocompatibility Complex (MHC) Expression: Influence on Histone Modifications and CBP Recruitment to the Class II MHC Promoter Mol. Endocrinol., August 1, 2004; 18(8): 1963 - 1974. [Abstract] [Full Text] [PDF]

				C. H. Selzman, J. S. Gaynor, A. S. Turner, S. M. Johnson, L. D. Horwitz, T. A. Whitehill, and A. H. Harken Ovarian Ablation Alone Promotes Aortic Intimal Hyperplasia and Accumulation of Fibroblast Growth Factor Circulation, November 10, 1998; 98(19): 2049 - 2054. [Abstract] [Full Text] [PDF]

				H. Lou, M. B. Martin, A. Stoica, P. W. Ramwell, and M. L. Foegh Upregulation of Estrogen Receptor-{alpha} Expression in Rabbit Cardiac Allograft Circ. Res., November 2, 1998; 83(9): 947 - 951. [Abstract] [Full Text] [PDF]

				T. J. K. Toung, R. J. Traystman, P. D. Hurn, and V. M. Miller Estrogen-Mediated Neuroprotection After Experimental Stroke in Male Rats • Editorial Comment Stroke, August 1, 1998; 29(8): 1666 - 1670. [Abstract] [Full Text] [PDF]