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J Thorac Cardiovasc Surg 1999;117:383-389
© 1999 Mosby, Inc.

CARDIOPULMONARY SUPPORT AND PHYSIOLOGY

ISCHEMIC PRECONDITIONING ATTENUATES POSTISCHEMIC CORONARY ARTERY ENDOTHELIAL DYSFUNCTION IN A MODEL OF MINIMALLY INVASIVE DIRECT CORONARY ARTERY BYPASS GRAFTING

From the Division of Cardiothoracic Surgery, Department of Surgery, Emory University School of Medicine, Carlyle Fraser Heart Center-Cardiothoracic Research Laboratory, Crawford Long Hospital, Atlanta.

Presented at the Seventieth Scientific Sessions, American Heart Association, Orlando, Florida, Nov 9-12, 1997.

Address for reprints: Jakob Vinten-Johansen, PhD, Cardiothoracic Research Laboratory, Crawford Long Hospital, 550 Peachtree St, NE, Atlanta, GA 30365-2225.

Objective: Unmodified reperfusion without cardioplegia in minimally invasive direct coronary artery bypass grafting procedures causes endothelial dysfunction that may predispose to polymorphonuclear neutrophil-mediated myocardial injury. This study tested the hypothesis that ischemic preconditioning in a minimally invasive direct coronary artery bypass grafting model attenuates postischemic endothelial dysfunction in coronary vessels.
Methods: In anesthetized dogs, the left anterior descending coronary artery was occluded for 30 minutes and reperfused for 3 hours without ischemic preconditioning (no-ischemic preconditioning; n = 7); in 7 dogs, the left anterior descending occlusion was preceded by 5 minutes occlusion followed by 5 minutes of reperfusion. Relaxation responses to stimulators of nitric oxide synthase were used to evaluate endothelial function in arteries from the ischemic-reperfused (left anterior descending) and nonischemic (left circumflex coronary artery) zones.
Results: Stimulated endothelial-dependent relaxation of epicardial left anterior descending artery to incremental concentrations of acetylcholine in the no-ischemic preconditioning animals was shifted to the right, and maximal relaxation was attenuated compared with the nonischemic left circumflex coronary artery (117% ± 4% vs 138% ± 5%). In contrast, acetylcholine-induced maximal relaxation was comparable in the left anterior descending artery versus left circumflex coronary artery in the ischemic preconditioning group (130% ± 6% vs 135% ± 5%). In 150- to 200-µm left anterior descending microvessels, 50% relaxation occurred with a lower concentration (log[M]) of acetylcholine in ischemic preconditioning versus no-ischemic preconditioning (–8.0 ± 0.4 vs –7.0 ± 0.1) with no group differences in smooth muscle relaxation to sodium nitroprusside, suggesting endothelial-specific damage. Adherence of fluorescent labeled polymorphonuclear neutrophils to epicardial coronary artery endothelium, used as an index of basal (unstimulated) anti–polymorphonuclear neutrophil function, was significantly attenuated by ischemic preconditioning versus no-ischemic preconditioning (293 ± 25 polymorphonuclear neutrophils/mm² vs 528 ± 29 polymorphonuclear neutrophils/mm²).
Conclusion: In this minimally invasive direct coronary artery bypass grafting model, both agonist-stimulated and basal postischemic endothelial dysfunction were attenuated by ischemic preconditioning.

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