HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Personal Folders Download to citation manager Author home page(s): Bassem N. Mora Permission Requests Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Yano, M. Articles by Patterson, G. A. Search for Related Content PubMed PubMed Citation Articles by Yano, M. Articles by Patterson, G. A.

J Thorac Cardiovasc Surg 1999;117:705-713
© 1999 Mosby, Inc.

CARDIOTHORACIC TRANSPLANTATION

EX VIVO TRANSFECTION OF TRANSFORMING GROWTH FACTOR-ß₁ GENE TO PULMONARY ARTERY SEGMENTS IN LUNG GRAFTS

From the Division of Cardiothoracic Surgery^a and the Department of Surgery, Department of Pathology,^b Washington University School of Medicine, St Louis, Mo, and Genzyme Corporation,^c Framingham, Mass.

Supported by National Institutes of Health grant 1R0l HL-41281.

Read at the Twenty-fourth Annual Meeting of The Western Thoracic Surgical Association, Whistler, British Columbia, June 24-27, 1998.

Received for publication June 26, 1998. Revisions requested Aug 20, 1998. Revisions received Dec 14, 1998. Accepted for publication Dec 15, 1998. Address for reprints: G. Alexander Patterson, MD, 3108 Queeny Tower, One Barnes-Jewish Hospital Plaza, St Louis, MO 63110.

Objective: Proximal pulmonary artery segment transfection may provide beneficial downstream effects on the whole-lung graft. In this study, transforming growth factor-ß₁ was transfected to proximal pulmonary artery segments, and the efficacy of transforming growth factor-ß₁ transfection was examined in ischemia-reperfusion injury and acute rejection models of rat lung transplantation.
Methods: In the ischemia-reperfusion injury model, orthotopic left lung transplantation was performed in F344 rats. In group I, the PPAS was isolated and injected with saline solution. In 2 other groups, lipid67:DOPE:sense (group II) or antisense transforming growth factor-ß₁pDNA construct (group III) was injected instead of saline solution. After cold preservation at 4°C for 18 hours, lung grafts were implanted. Graft function was assessed 24 hours later. In the acute rejection model, donor lung grafts were harvested. Proximal pulmonary artery segments were injected with saline solution (group I) or sense (group II) or antisense lipid gene construct (group III) and then implanted. Graft function was assessed on postoperative day 5.
Results: In the ischemia-reperfusion injury study, there were no significant differences in oxygenation, wet-to-dry weight ratios, graft myeloperoxidase activity, or transforming growth factor-ß₁ levels in platelet-poor serum or proximal pulmonary artery segment homogenates. In the acute rejection study, oxygenation was significantly improved in group II receiving transforming growth factor-ß₁ (group II vs I and III, 136.0 ± 32.5 vs 54.0 ± 9.6 mm Hg and 53.8 ± 14.8 mm Hg; P = .016 and .016). There were no significant pathologic differences. Transforming growth factor-ß₁ concentrations from proximal pulmonary artery segment homogenates in group II were significantly higher compared with controls.
Conclusions: Ex vivo transfection of transforming growth factor-ß₁ to proximal pulmonary artery segments did not affect reperfusion injury of lung isografts. In acute rejection, however, ex vivo transfection of transforming growth factor-ß ₁ to proximal pulmonary artery segments improved allograft function. This suggests that transfection to proximal pulmonary artery segments exerts beneficial downstream effects on the whole-lung allograft. (J Thorac Cardiovasc Surg 1999; 117:705-13)

This article has been cited by other articles:

				K. Ohmori, S.-i. Takeda, S. Miyoshi, M. Minami, S. Nakane, M. Ohta, Y. Sawa, and H. Matsuda Application of HVJ-liposome mediated gene transfer in lung transplantation--distribution and transfection efficiency in the lung Eur. J. Cardiothorac. Surg., May 1, 2005; 27(5): 768 - 773. [Abstract] [Full Text] [PDF]

				K. Ohmori, S.-i. Takeda, S. Miyoshi, M. Minami, S. Nakane, M. Ohta, Y. Sawa, and H. Matsuda Attenuation of lung injury in allograft rejection using NF-{kappa}B decoy transfection--novel strategy for use in lung transplantation Eur. J. Cardiothorac. Surg., January 1, 2005; 27(1): 23 - 27. [Abstract] [Full Text] [PDF]

				T. Suda, T. Tagawa, S. A. Kanaan, B. D. Kozower, N. Daddi, T. Mohanakumar, and G. A. Patterson Adenovirus encoding soluble tumor necrosis factor {alpha} receptor immunoglobulin prolongs gene expression of a cotransfected reporter gene in rat lung J. Thorac. Cardiovasc. Surg., October 1, 2003; 126(4): 1155 - 1161. [Abstract] [Full Text] [PDF]

				M. de Perrot, M. Liu, T. K. Waddell, and S. Keshavjee Ischemia-Reperfusion-induced Lung Injury Am. J. Respir. Crit. Care Med., February 15, 2003; 167(4): 490 - 511. [Abstract] [Full Text] [PDF]

				T. Tagawa, T. Suda, N. Daddi, B. D. Kozower, S. A. Kanaan, T. Mohanakumar, and G. A. Patterson Low-dose endobronchial gene transfer to ameliorate lung graft ischemia-reperfusion injury J. Thorac. Cardiovasc. Surg., April 1, 2002; 123(4): 795 - 802. [Abstract] [Full Text] [PDF]

				T. Suda, F. D'Ovidio, N. Daddi, J. H. Ritter, T. Mohanakumar, and G. A. Patterson Recipient intramuscular gene transfer of active transforming growth factor-{beta}1 attenuates acute lung rejection Ann. Thorac. Surg., May 1, 2001; 71(5): 1651 - 1656. [Abstract] [Full Text] [PDF]

				B. N. Mora, C. H. R. Boasquevisque, M. Boglione, J. M. Ritter, R. K. Scheule, N. S. Yew, L. Debruyne, L. Qin, J. S. Bromberg, and G. A. Patterson Transforming growth factor-{beta}1 gene transfer ameliorates acute lung allograft rejection J. Thorac. Cardiovasc. Surg., May 1, 2000; 119(5): 913 - 920. [Abstract] [Full Text] [PDF]

				T. Suda, B. N. Mora, F. D’Ovidio, J. A. Cooper, M. Hiratsuka, W. Zhang, T. Mohanakumar, and G. A. Patterson IN VIVO ADENOVIRUS-MEDIATED ENDOTHELIAL NITRIC OXIDE SYNTHASE GENE TRANSFER AMELIORATES LUNG ALLOGRAFT ISCHEMIA-REPERFUSION INJURY J. Thorac. Cardiovasc. Surg., February 1, 2000; 119(2): 297 - 304. [Abstract] [Full Text] [PDF]

				M. Yano, M. Hiratsuka, B. N. Mora, R. K. Scheule, and G. A. Patterson Transfection of pulmonary artery segments in lung isografts during storage Ann. Thorac. Surg., November 1, 1999; 68(5): 1810 - 1814. [Abstract] [Full Text] [PDF]

				F. D’Ovidio, M. Yano, J. H. Ritter, T. Mohanakumar, and G. A. Patterson Endobronchial transfection of naked TGF-{beta}1 cDNA attenuates acute lung rejection Ann. Thorac. Surg., September 1, 1999; 68(3): 1008 - 1013. [Abstract] [Full Text] [PDF]