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J Thorac Cardiovasc Surg 2000;119:324-330
© 2000 Mosby, Inc.

SURGERY FOR CONGENITAL HEART DISEASE

CLASS I AND CLASS II ANTI-HLA ANTIBODIES AFTER IMPLANTATION OF CRYOPRESERVED ALLOGRAFT MATERIAL IN PEDIATRIC PATIENTS

From the Departments of Surgery, Pathology, and Pediatrics, Primary Children’s Medical Center and the University of Utah, Salt Lake City, Utah.

Address for reprints: John A. Hawkins, MD, Pediatric Cardiothoracic Surgery, Primary Children’s Medical Center, 100 North Medical Dr, Salt Lake City, UT 84113 (E-mail: jhawkins{at}med.utah.edu) .

Objectives: Very little is known regarding the immune response to cryopreserved allograft valves and patch material used in the surgical repair of congenital heart defects.
Methods: We prospectively measured the frequency of panel reactive antibodies directed against HLA class I (HLA-A, B, and C) and class II (HLA-DR/DQ) alloantigens in 24 children receiving cryopreserved allografts. We compared them with results in 11 previously reported control patients. Sixteen of the study patients underwent placement of a valved conduit (11 pulmonic, 5 aortic) between the right ventricle and pulmonary arteries, 6 underwent patch angioplasty of stenotic vessels with cryopreserved pulmonary artery, and 2 underwent placement of a pulmonary monocusp patch. Study patients had panel reactive antibodies measured before, 1 month, 3 months, and 1 year after the operation.
Results: With allograft implantation, panel reactive antibodies increased from 1.9% ± 5% before the operation to 62% ± 33% at 31 ± 8 days after the operation, 92% ± 15% at 3.3 ± 0.6 months after the operation, and 85% ± 18% at 1.1 ± 0.2 years after the operation. The control group showed no change in panel reactive antibodies, with a level of 1.6% ± 1% before the operation, 3.2% ± 1% 28 ± 5 days after the operation, and 1.7% ± 1% 2.7 ± 0.3 months after the operation. Class II antibodies (anti-HLA-DR/DQ) rose to 49% ± 35% at 30 ± 8 days and 70% ± 26% at 3.3 ± 0.6 months after the operation.
Conclusions: Cryopreserved allograft material induces a marked response that involves both class I and class II anti-HLA antibodies within 3 months after operation in children. This alloantibody response may represent a form of "rejection," may have implications for those who require subsequent cardiac transplantation, and may play a role in early allograft failure.

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