| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J Thorac Cardiovasc Surg 2000;119:732-740
© 2000 The American Association for Thoracic Surgery
SURGERY FOR CONGENITAL HEART DISEASE |
From the Department of Cardiovascular Surgery,a Department of Surgery,b Department of Cardiology,c Children’s Hospital, Boston, Mass; the Department of Pathology,d Brigham and Women’s Hospital, Boston, Mass; Metabolix Inc,e Cambridge, Mass; and the Department of Chemical Engineering,f Massachusetts Institute of Technology, Cambridge, Mass.
Supported by grants from the Department of Cardiac Surgery, Children’s Hospital, Boston, Mass; Deutsche Forschungsgemeinschaft (Sto 359/1-1); and the National Institutes of Health (HL-97-005).
Address for reprints: John E. Mayer, Jr, MD, Department of Cardiovascular Surgery, Children’s Hospital Harvard Medical School, 300 Longwood Ave, Boston, MA 02115 (E-mail: mayer{at}a1.tch-harvard.edu ).
Objective: Bioprosthetic and mechanical valves and valved conduits are unable to grow, repair, or remodel. In an attempt to overcome these shortcomings, we have evaluated the feasibility of creating 3-leaflet, valved, pulmonary conduits from autologous ovine vascular cells and biodegradable polymers with tissue-engineering techniques.
Methods: Endothelial cells and vascular medial cells were harvested from ovine carotid arteries. Composite scaffolds of polyglycolic acid and polyhydroxyoctanoates were formed into a conduit, and 3 leaflets (polyhydroxyoctanoates) were sewn into the conduit. These constructs were seeded with autologous medial cells on 4 consecutive days and coated once with autologous endothelial cells. Thirty-one days (±3 days) after cell harvesting, 8 seeded and 1 unseeded control constructs were implanted to replace the pulmonary valve and main pulmonary artery on cardiopulmonary bypass. No postoperative anticoagulation was given. Valve function was assessed by means of echocardiography. The constructs were explanted after 1, 2, 4, 6, 8, 12, 16, and 24 weeks and evaluated macroscopically, histologically, and biochemically.
Results: Postoperative echocardiography of the seeded constructs demonstrated no thrombus formation with mild, nonprogressive, valvular regurgitation up to 24 weeks after implantation. Histologic examination showed organized and viable tissue without thrombus. Biochemical assays revealed increasing cellular and extracellular matrix contents. The unseeded construct developed thrombus formation on all 3 leaflets after 4 weeks.
Conclusion: This experimental study showed that valved conduits constructed from autologous cells and biodegradable matrix can function in the pulmonary circulation. The progressive cellular and extracellular matrix formation indicates that the remodeling of the tissue-engineered structure continues for at least 6 months.
This article has been cited by other articles:
|
|
 |
|
  J. E. Mayer Tissue Engineering for Cardiac Valve Surgery Card. Surg. Adult, January 1, 2008; 3(2008): 1649 - 1656. [Full Text]
|
|
|
|
 |
|
 V. L. Sales, G. C. Engelmayr Jr, J. A. Johnson Jr, J. Gao, Y. Wang, M. S. Sacks, and J. E. Mayer Jr Protein Precoating of Elastomeric Tissue-Engineering Scaffolds Increased Cellularity, Enhanced Extracellular Matrix Protein Production, and Differentially Regulated the Phenotypes of Circulating Endothelial Progenitor Cells Circulation, September 11, 2007; 116(11_suppl): I-55 - I-63. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Vincentelli, F. Wautot, F. Juthier, O. Fouquet, D. Corseaux, S. Marechaux, T. Le Tourneau, O. Fabre, S. Susen, E. Van Belle, et al. In vivo autologous recellularization of a tissue-engineered heart valve: Are bone marrow mesenchymal stem cells the best candidates? J. Thorac. Cardiovasc. Surg., August 1, 2007; 134(2): 424 - 432. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Hayashida, K. Kanda, H. Yaku, J. Ando, and Y. Nakayama Development of an in vivo tissue-engineered, autologous heart valve (the biovalve): Preparation of a prototype model J. Thorac. Cardiovasc. Surg., July 1, 2007; 134(1): 152 - 159. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Juthier, A. Vincentelli, J. Gaudric, D. Corseaux, O. Fouquet, C. Calet, T. L. Tourneau, V. Soenen, C. Zawadzki, O. Fabre, et al. Decellularized heart valve as a scaffold for in vivo recellularization: Deleterious effects of granulocyte colony-stimulating factor J. Thorac. Cardiovasc. Surg., April 1, 2006; 131(4): 843 - 852. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. G. Leyh, M. Wilhelmi, P. Rebe, S. Ciboutari, A. Haverich, and H. Mertsching Tissue Engineering of Viable Pulmonary Arteries for Surgical Correction of Congenital Heart Defects Ann. Thorac. Surg., April 1, 2006; 81(4): 1466 - 1471. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Rieder, G. Seebacher, M.-T. Kasimir, E. Eichmair, B. Winter, B. Dekan, E. Wolner, P. Simon, and G. Weigel Tissue Engineering of Heart Valves: Decellularized Porcine and Human Valve Scaffolds Differ Importantly in Residual Potential to Attract Monocytic Cells Circulation, May 31, 2005; 111(21): 2792 - 2797. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. F. Corno, S. D. Qanadli, N. Sekarski, S. Artemisia, M. Hurni, P. Tozzi, and L. K. von Segesser Bovine Valved Xenograft in Pulmonary Position: Medium-Term Follow-Up With Excellent Hemodynamics and Freedom From Calcification Ann. Thorac. Surg., October 1, 2004; 78(4): 1382 - 1388. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Iwai, Y. Sawa, H. Ichikawa, S. Taketani, E. Uchimura, G. Chen, M. Hara, J. Miyake, and H. Matsuda Biodegradable polymer with collagen microsponge serves as a new bioengineered cardiovascular prosthesis J. Thorac. Cardiovasc. Surg., September 1, 2004; 128(3): 472 - 479. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Kadner, G. Zund, C. Maurus, C. Breymann, S. Yakarisik, G. Kadner, M. Turina, and S. P. Hoerstrup Human umbilical cord cells for cardiovascular tissue engineering: a comparative study Eur. J. Cardiothorac. Surg., April 1, 2004; 25(4): 635 - 641. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Schenke-Layland, F. Opitz, M. Gross, C. Doring, K.J. Halbhuber, F. Schirrmeister, Th. Wahlers, and U.A. Stock Complete dynamic repopulation of decellularized heart valves by application of defined physical signals--an in vitro study Cardiovasc Res, December 1, 2003; 60(3): 497 - 509. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. G. Leyh, M. Wilhelmi, T. Walles, K. Kallenbach, P. Rebe, A. Oberbeck, T. Herden, A. Haverich, and H. Mertsching Acellularized porcine heart valve scaffolds for heart valve tissue engineering and the risk of cross-species transmission of porcine endogenous retrovirus J. Thorac. Cardiovasc. Surg., October 1, 2003; 126(4): 1000 - 1004. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Q. Zhou, A. F. Corno, C. H. Huber, P. Tozzi, and L. K. von Segesser Self-expandable valved stent of large size: off-bypass implantation in pulmonary position Eur. J. Cardiothorac. Surg., August 1, 2003; 24(2): 212 - 216. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. G. Leyh, M. Wilhelmi, P. Rebe, S. Fischer, T. Kofidis, A. Haverich, and H. Mertsching In vivo repopulation of xenogeneic and allogeneic acellular valve matrix conduits in the pulmonary circulation Ann. Thorac. Surg., May 1, 2003; 75(5): 1457 - 1463. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. W. H. Sutherland and J. E. Mayer Jr. Tissue engineering for cardiac surgery Card. Surg. Adult, January 1, 2003; 2(2003): 1527 - 1536. [Full Text]
|
|
|
|
|
|
T. Ozawa, D. A. G. Mickle, R. D. Weisel, N. Koyama, H. Wong, S. Ozawa, and R.-K. Li Histologic changes of nonbiodegradable and biodegradable biomaterials used to repair right ventricular heart defects in rats J. Thorac. Cardiovasc. Surg., December 1, 2002; 124(6): 1157 - 1164. [Abstract] [Full Text]
|
|
|
|
|
|
A. Kadner, S. P. Hoerstrup, J. Tracy, C. Breymann, C. F. Maurus, S. Melnitchouk, G. Kadner, G. Zund, and M. Turina Human umbilical cord cells: a new cell source for cardiovascular tissue engineering Ann. Thorac. Surg., October 1, 2002; 74(4): S1422 - 1428. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. P. Hoerstrup, A. Kadner, S. Melnitchouk, A. Trojan, K. Eid, J. Tracy, R. Sodian, J. F. Visjager, S. A. Kolb, J. Grunenfelder, et al. Tissue Engineering of Functional Trileaflet Heart Valves From Human Marrow Stromal Cells Circulation, September 24, 2002; 106(12_suppl_1): I-143 - I-150. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Ozawa, D. A. G. Mickle, R. D. Weisel, N. Koyama, S. Ozawa, and R.-K. Li Optimal Biomaterial for Creation of Autologous Cardiac Grafts Circulation, September 24, 2002; 106(12_suppl_1): I-176 - I-182. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Jux, H. Bertram, P. Wohlsein, M. Brugmann, P. Wuboldt, C. Fink, T. Paul, and G. Hausdorf Experimental ASD closure using autologous cell-seeded interventional closure devices Cardiovasc Res, January 1, 2002; 53(1): 181 - 191. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. R. Fuchs, B. A. Nasseri, and J. P. Vacanti Tissue engineering: a 21st century solution to surgical reconstruction Ann. Thorac. Surg., August 1, 2001; 72(2): 577 - 591. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. A. Stock, T. Sakamoto, S. Hatsuoka, D. P. Martin, M. Nagashima, A. M. Moran, M. A. Moses, P. N. Khalil, F. J. Schoen, J. P. Vacanti, et al. Patch augmentation of the pulmonary artery with bioabsorbable polymers and autologous cell seeding J. Thorac. Cardiovasc. Surg., December 1, 2000; 120(6): 1158 - 1167. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| ANN THORAC SURG | ASIAN CARDIOVASC THORAC ANN | EUR J CARDIOTHORAC SURG |
| J THORAC CARDIOVASC SURG | ICVTS | ALL CTSNet JOURNALS |
