HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Personal Folders Download to citation manager Permission Requests Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Liu, Y. Articles by Shimizu, Y. Search for Related Content PubMed PubMed Citation Articles by Liu, Y. Articles by Shimizu, Y.

J Thorac Cardiovasc Surg 2000;120:108-114
© 2000 The American Association for Thoracic Surgery

GENERAL THORACIC SURGERY

Immunosuppressant-free allotransplantation of the tracheaThe antigenicity of tracheal grafts can be reduced by removing the epithelium and mixed glands from the graft by detergent treatment

From the Department of Bioartificial Organs, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan.

Address for reprints: Yu Liu, MD, Department of Bioartificial Organs, Institute for Frontier Medical Sciences, Kyoto University, 53 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan (E-mail: lyu{at}frontier.kyoto-u.ac.jp ).

Objective: To develop a method for eliminating the epithelium and mixed glands from tracheal grafts by detergent treatment and evaluate these grafts for immunosuppressant-free allotransplantation in dogs.
Methods: Fresh canine tracheal grafts were treated with a detergent (1% Triton X-100 t-octylphenoxypolyethoxyethanol; T-9284; Sigma Chemical Co, St Louis, Mo) at 4°C for 48 hours. The grafts were then used for intrathoracic 5-ring tracheal replacement in other dogs without immunosuppressant treatment (n = 6, detergent treatment group). In the control group (n = 6) fresh untreated canine tracheal segments were implanted as allografts. All the implanted grafts were covered with an omental pedicle.
Results: In the detergent treatment group the chondrocytes in the graft had a similar appearance to those in the fresh trachea, indicating that the chondrocytes remained viable after the detergent treatment. In 5 of the 6 grafts, the epithelium and mixed glands had been removed completely. After transplantation, these 5 grafts were incorporated by the host trachea without stenosis. In the remaining treated tracheal graft, in which removal of the epithelium was incomplete, moderate stenosis was observed at the fourth week after implantation, although this was not progressive. In the control group, granulation tissue of the graft and significant stenosis were observed after transplantation.
Conclusion: The antigenicity of tracheal grafts can be greatly reduced by removing the epithelium and mixed glands by the use of detergent treatment. The epithelium and mixed glands of the graft appear to be the determining elements involved in rejection after tracheal allotransplantation.

This article has been cited by other articles:

				E. A. Agathos, P. Tomos, E. Lachanas, H. Gakiopoulou, A. Pantopoulou, and D. Perrea Experimental Replacement of Pig Trachea with Novel Bioprosthesis from Harp Seal Asian Cardiovascular and Thoracic Annals, December 1, 2010; 18(6): 557 - 562. [Abstract] [Full Text] [PDF]

				T. Iyikesici, B. Tuncozgur, M. Sanli, A. F. Isik, F. Meteroglu, and L. Elbeyli Two-piece cryopreserved tracheal allotransplantation: an experimental study Eur J Cardiothorac Surg, October 1, 2009; 36(4): 722 - 726. [Abstract] [Full Text] [PDF]

				L. Yang, S. Korom, M. Welti, S. P. Hoerstrup, G. Zund, F. J. Jung, P. Neuenschwander, and W. Weder Tissue engineered cartilage generated from human trachea using DegraPol(R) scaffold Eur J Cardiothorac Surg, August 1, 2003; 24(2): 201 - 207. [Abstract] [Full Text] [PDF]

				E. Stoelben, H. Harpering, J. Haberstroh, A. di Filippo, and E. Wellens Heterotopic transplantation of cryopreserved tracheae in a rat model Eur J Cardiothorac Surg, January 1, 2003; 23(1): 15 - 20. [Abstract] [Full Text] [PDF]

				M. Behrend, R. von Wasielewski, and J. Klempnauer Failure of airway healing in an ovine autotransplantation model that includes basic fibroblast growth factor J. Thorac. Cardiovasc. Surg., August 1, 2002; 124(2): 231 - 240. [Abstract] [Full Text] [PDF]

				H. C. Grillo Tracheal replacement: a critical review Ann. Thorac. Surg., June 1, 2002; 73(6): 1995 - 2004. [Abstract] [Full Text] [PDF]

				T. Murakawa, J. Nakajima, N. Motomura, A. Murakami, and S. Takamoto Successful allotransplantation of cryopreserved tracheal grafts with preservation of the pars membranacea in nonhuman primates J. Thorac. Cardiovasc. Surg., January 1, 2002; 123(1): 153 - 160. [Abstract] [Full Text] [PDF]

				Y. Liu, T. Nakamura, Y. Shimizu, H. Ueda, M. Yoshitani, T. Toba, and S. Fukuda Tracheal allotransplantation in beagle dogs without immunosuppressants Ann. Thorac. Surg., October 1, 2001; 72(4): 1190 - 1194. [Abstract] [Full Text] [PDF]