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J Thorac Cardiovasc Surg 2001;121:1187-1193
© 2001 The American Association for Thoracic Surgery

Evolving Technology

Transplantation of tracheal epithelial cells onto a prefabricated capsule pouch with fibrin glue as a delivery vehicle

From the Department of Plastic and Reconstructive Surgery, Leopold-Franzens University, and the Ludwig Boltzmann Institute for Quality Control in Plastic and Reconstructive Surgery, Innsbruck, Austria^a; and the Institute for Plastic and Reconstructive Surgery, Department of Surgery, Southern Illinois University School of Medicine, Springfield, Ill.^b

Presented at the Second BioValley Tissue Engineering Symposium, Freiburg, Germany, November 25, 1999, and the Eleventh Annual Meeting of the European Association of Plastic Surgeons, Berlin, Germany, June 3, 2000.

Received for publication Aug 30, 2000 Revisions requested Dec 6, 2000; revisions received Dec 21, 2000. Accepted for publication Dec 27, 2000. Address for reprints: Christian Rainer, MD, Universit^_ats- Klinik für Plastische und Wiederherstellungschirurgie Anichstrasse 35, A-6020 Innsbruck, Austria (E-mail: christian.rainer{at}chello.at).

Objective: The purpose of this study was to investigate whether in vitro cultured tracheal epithelial cells can be transplanted onto a prefabricated capsule surface in vivo for possible use in tracheal reconstruction.
Methods: Tracheal epithelial cells from 12 donor inbred rats were harvested for culture and expansion. In 16 recipient inbred rats, 2 sterile cylinders made of silicone rubber were implanted in each rat bilaterally in the folds of both the left and right anterior rectus sheath by wrapping the sheaths around the cylinders to induce a capsule formation. Ten days later, the cell cultures were divided and suspended in 1 of 2 delivery vehicles (standard culture medium or fibrin glue) and implanted onto the capsule surface. To compare the 2 delivery vehicles, we used fibrin glue on one side and the standard culture medium on the other.
Results: After 2 (group 1, n = 8) and 4 (group 2, n = 8) weeks, histologic findings, immunohistochemical staining, and electron microscopy demonstrated the capsule to be covered with a tracheal neoepithelium in group 1 and additional ciliated cells and secretory cells in a confluent layer in group 2 but only on the side with fibrin glue as the delivery vehicle. No viable epithelial cells were identified on the side with the standard culture medium in either group.
Conclusion: We conclude that cultured epithelial cells can be successfully transplanted onto a prefabricated capsule surface with fibrin glue, which will differentiate into morphologic, nearly normal epithelium, showing potential for tracheal reconstruction.

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