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J Thorac Cardiovasc Surg 2001;122:18-23
© 2001 The American Association for Thoracic Surgery

General Thoracic Surgery (GTS)

Lung retrieval from non–heart beating cadavers with the use of a rat lung transplant model

From the Division of Cardiothoracic Surgery, University of North Carolina School of Medicine, Chapel Hill, NC.

Received for publication June 12, 2000. Revisions requested July 27, 2000; revisions received Jan 5, 2001. Accepted for publication Jan 26, 2001. Address for reprints: Thomas M. Egan, MD, 108 Burnett-Womack Building, CB #7065, Chapel Hill, NC 27599-7065 (E-mail: ltxtme{at}med.unc.edu).

Abstract

Background: Lungs retrieved from cadavers after death and circulatory arrest may alleviate the critical shortage of lungs for transplant. We report a rat lung transplantation model that allows serial measurement of arterial blood gases after left single lung transplantation from non–heart beating donors.
Methods: Twelve Sprague-Dawley rats underwent left lung transplantation with a vascular cuff technique. Donor rats were anesthetized with intraperitoneal injection of pentobarbital, heparinized, intubated via tracheotomy, and then killed with pentobarbital. Lungs were retrieved immediately or after 2 hours of oxygen ventilation after death (tidal volume 1 mL/100 g, rate 40/min FIO₂ = 1.0, positive end-expiratory pressure 5 cm H₂O). Recipient rats were anesthetized, intubated, and ventilated. The carotid artery and jugular vein were cannulated for arterial blood gases and infusion of Ringer's lactate (4 mL/h). Anesthesia was maintained with halothane 0.2%, and recipient arterial blood gases were measured at 4 and 6 hours after lung transplantation after snaring the right pulmonary artery for 5 minutes. Animals were put to death 6 hours after lung transplantation, and portions of transplanted lungs were frozen in liquid nitrogen and assayed for wet/dry ratio, myeloperoxidase as a measure of neutrophil infiltration, and conjugated dienes as a measure of free radical–mediated lipid peroxidation.
Results: Arterial PO₂ and wet/dry ratio were not significantly different in recipients of non–heart beating donor lungs retrieved immediately after death or after 2 hours of oxygen ventilation. Significant neutrophil infiltration was observed in recipients of non–heart beating donor lungs retrieved 2 hours after death from oxygenventilated donors.
Conclusions: Strategies to ameliorate reperfusion injury may allow for successful lung transplantation from non–heart beating donors.

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