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Right arrow Transplantation - heart

J Thorac Cardiovasc Surg 2001;122:508-517
© 2001 The American Association for Thoracic Surgery

Cardiothoracic Transplantation (TX)

Vascular cell adhesion molecule-1 up-regulation and phenotypic modulation of vascular smooth muscle cells predate mononuclear infiltration in transplant arteriopathy

Sadahiro Sai, MDa, Akiko Iwata, PhDb, Robert Thomas, BAb, Margaret D. Allen, MD, DrSc(Hon)c

From the Department of Cardiovascular Surgery,a Tohoku University, Sendai, Japan, the University of Washington,b Seattle, and The Hope Heart Institute,c Seattle, Wash.

Received for publication June 22, 2000. Revisions requested Oct 16, 2000; revisions received Nov 21, 2000. Accepted for publication Dec 11, 2000. Address for reprints: Margaret D. Allen, MD, The Hope Heart Institute, 1124 Columbia St, Suite 120, Seattle, WA 98104 (E-mail: mdallenmd{at}yahoo.com).

Abstract

Objective: Expression of embryonic myosin heavy chain isoforms and vascular cell adhesion molecule-1 by neointimal vascular smooth muscle cells are independent indicators of atherosclerotic plaque development in both human beings and experimental animal models. We examined the chronologic change in smooth muscle cell myosin heavy chain isoforms, vascular cell adhesion molecule-1 expression, and mononuclear cell infiltration in a carotid arterial transplant model to ascertain whether similar phenotypic changes would occur in transplant arteriopathy.
Methods: Transplanted rabbit carotid arteries were examined at 7, 14, 21, and 35 days (n = 5, 7, 6, and 5, respectively). Lesion progression and the prevalence of smooth muscle cell myosin heavy chain isoforms, T-lymphocytes, macrophages, and vascular cell adhesion molecule-1 expression were evaluated immunohistochemically by computerized image analysis.
Results: In this carotid arterial transplant model, the intima/media area ratio increased significantly by 35 days (P = .01) as cell density decreased (P = .01), suggesting extracellular matrix elaboration. Intimal smooth muscle cells expressing embryonic phenotypes were seen as early as 7 days, a phenotypic change that predated mononuclear cell infiltration of the graft by at least 7 days. By 35 days, up to 70% of intimal smooth muscle cells expressed the embryonic phenotype, coinciding with the transition from inflammatory to chronic lesions. Although, in early lesions, vascular cell adhesion molecule-1 was identified on luminal endothelium overlying mononuclear infiltrates, in advanced lesions vascular cell adhesion molecule-1 was identified primarily on intimal vascular smooth muscle cells.
Conclusions: Overall, these vascular smooth muscle cell changes mark important early events in transplant arteriopathy that may not be ameliorated by immunosuppressive regimens in routine use.






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