| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J Thorac Cardiovasc Surg 2001;122:963-971
© 2001 The American Association for Thoracic Surgery
Cardiopulmonary Support and Physiology (CSP) |
From the Division of Cardiovascular Surgery, Toronto General Hospital, Toronto General Research Institute, University Health Network, Department of Surgery, University of Toronto, Toronto, Ontario, Canada.
R.K.L. is a Career Investigator of the Heart and Stroke Foundation of Canada. R.D.W. was a Career Investigator of the Heart and Stroke Foundation of Ontario. This research was supported by research grants to R.K.L. from the Medical Research Council of Canada (MT-13665).
Received for publication Jan 17, 2001. Revisions requested May 8, 2001. Accepted for publication May 8, 2001. Address for reprints: Ren-Ke Li, MD, PhD, Department of Cardiovascular Surgery, Toronto General Hospital, CCRW 1-815, 200 Elizabeth St, Toronto, Ontario, Canada M5G 2C4 (E-mail: RenKe{at}UHN.on.ca).
Abstract
Purpose: Myocardial angiogenesis may improve regional perfusion and perhaps function after cardiac injury. We evaluated the effect of endothelial cell transplantation into a myocardial scar on angiogenesis and ventricular function, as an alternative to angiogenic gene or protein therapy.
Methods and Results: A transmural myocardial scar was created in the left ventricular free wall of rat hearts by cryoinjury. Allogeneic aortic endothelial cells were injected into the scar 2 weeks after cryoinjury. A cluster of transplanted cells was identified at the site of injection 1 day and 1 week after transplantation, but not after 2 weeks. The size of this cluster of transplanted cells decreased as vascular density in the transplanted scar tissue increased with time. Six weeks after transplantation, vascular density was significantly greater in transplanted hearts than in control hearts. Regional blood flow, by microsphere analysis, was greater in the transplanted rats. Systolic and diastolic ventricular function was similar between groups. In a second series of experiments, syngeneic aortic endothelial cells labeled with bromodeoxyuridine were transplanted 2 weeks after cryoinjury. Vascular density in the transplanted scar was greater than in controls. Labeled transplanted endothelial cells were identified forming part of the newly developed blood vessels. No difference in vascular density was found between allogeneic and syngeneic cell transplantation. Vascular endothelial growth factor was not expressed at greater levels in the transplanted cells or the myocardial scar.
Conclusion: Transplanted endothelial cells stimulated angiogenesis, were incorporated into the new vessels, and increased regional perfusion in myocardial scar tissue, but did not improve global function in this cryoinjury rat model.
Related Article
J. Thorac. Cardiovasc. Surg. 2001 122: 851-852.
This article has been cited by other articles:
|
|
 |
|
  P. Farahmand, T. Y.Y. Lai, R. D. Weisel, S. Fazel, T. Yau, P. Menasche, and R.-K. Li Skeletal Myoblasts Preserve Remote Matrix Architecture and Global Function When Implanted Early or Late After Coronary Ligation Into Infarcted or Remote Myocardium Circulation, September 30, 2008; 118(14_suppl_1): S130 - S137. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Kim, R.-K. Li, G. Li, Y. Zhang, R. D. Weisel, and T. M. Yau Effects of Cell-Based Angiogenic Gene Therapy at 6 Months: Persistent Angiogenesis and Absence of Oncogenicity Ann. Thorac. Surg., February 1, 2007; 83(2): 640 - 646. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M.-L. Huang, H. Tian, J. Wu, K. Matsubayashi, R. D. Weisel, and R.-K. Li Myometrial cells induce angiogenesis and salvage damaged myocardium Am J Physiol Heart Circ Physiol, November 1, 2006; 291(5): H2057 - H2066. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Hata, G. Matsumiya, S. Miyagawa, H. Kondoh, N. Kawaguchi, N. Matsuura, T. Shimizu, T. Okano, H. Matsuda, and Y. Sawa Grafted skeletal myoblast sheets attenuate myocardial remodeling in pacing-induced canine heart failure model J. Thorac. Cardiovasc. Surg., October 1, 2006; 132(4): 918 - 924. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Ye, H. K. Haider, and E. K. W. Sim Adult Stem Cells for Cardiac Repair: A Choice Between Skeletal Myoblasts and Bone Marrow Stem Cells Experimental Biology and Medicine, January 1, 2006; 231(1): 8 - 19. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. M. Yau, C. Kim, D. Ng, G. Li, Y. Zhang, R. D. Weisel, and R.-K. Li Increasing Transplanted Cell Survival With Cell-Based Angiogenic Gene Therapy Ann. Thorac. Surg., November 1, 2005; 80(5): 1779 - 1786. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Zhang, S. Fazel, H. Tian, D. A. G. Mickle, R. D. Weisel, T. Fujii, and R.-K. Li Increasing donor age adversely impacts beneficial effects of bone marrow but not smooth muscle myocardial cell therapy Am J Physiol Heart Circ Physiol, November 1, 2005; 289(5): H2089 - H2096. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Elmadbouh, Y. Chen, L. Louedec, S. Silberman, B. Pouzet, O. Meilhac, and J.-B. Michel Mesothelial cell transplantation in the infarct scar induces neovascularization and improves heart function Cardiovasc Res, November 1, 2005; 68(2): 307 - 317. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Nishiyama, K. Takaji, K. Kataoka, Y. Kurihara, M. Yoshimura, A. Kato, H. Ogawa, and H. Kurihara Id1 Gene Transfer Confers Angiogenic Property on Fully Differentiated Endothelial Cells and Contributes to Therapeutic Angiogenesis Circulation, November 1, 2005; 112(18): 2840 - 2850. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Mizuno, T. M. Yau, R. D. Weisel, C. G. Kiani, and R.-K. Li Elastin Stabilizes an Infarct and Preserves Ventricular Function Circulation, August 30, 2005; 112(9_suppl): I-81 - I-88. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Azarnoush, A. Maurel, L. Sebbah, C. Carrion, A. Bissery, C. Mandet, J. Pouly, P. Bruneval, A. A. Hagege, and P. Menasche Enhancement of the functional benefits of skeletal myoblast transplantation by means of coadministration of hypoxia-inducible factor 1{alpha} J. Thorac. Cardiovasc. Surg., July 1, 2005; 130(1): 173 - 179. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. M. Yau, G. Li, Y. Zhang, R. D. Weisel, D. A.G. Mickle, and R.-K. Li Vascular Endothelial Growth Factor Receptor Upregulation in Response to Cell-Based Angiogenic Gene Therapy Ann. Thorac. Surg., June 1, 2005; 79(6): 2056 - 2063. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Schuh, S. Breuer, R. Al Dashti, N. Sulemanjee, P. Hanrath, C. Weber, B. F. Uretsky, and E. R. Schwarz Administration of Vascular Endothelial Growth Factor Adjunctive to Fetal Cardiomyocyte Transplantation and Improvement of Cardiac Function in the Rat Model Journal of Cardiovascular Pharmacology and Therapeutics, January 1, 2005; 10(1): 55 - 66. [Abstract] [PDF]
|
|
|
|
|
|
T.-B. Liu, P. W. M. Fedak, R. D. Weisel, T. Yasuda, G. Kiani, D. A. G. Mickle, Z.-Q. Jia, and R.-K. Li Enhanced IGF-1 expression improves smooth muscle cell engraftment after cell transplantation Am J Physiol Heart Circ Physiol, December 1, 2004; 287(6): H2840 - H2849. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. C. Chachques, F. Duarte, B. Cattadori, A. Shafy, N. Lila, G. Chatellier, J.-N. Fabiani, and A. F. Carpentier Angiogenic growth factors and/or cellular therapy for myocardial regeneration: A comparative study J. Thorac. Cardiovasc. Surg., August 1, 2004; 128(2): 245 - 253. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. L. March and B. H. Johnstone Cellular approaches to tissue repair in cardiovascular disease: the more we know, the more there is to learn Am J Physiol Heart Circ Physiol, August 1, 2004; 287(2): H458 - H463. [Full Text] [PDF]
|
|
|
|
 |
|
 H. Fujii, S. Tomita, T. Nakatani, S. Fukuhara, A. Hanatani, Y. Ohtsu, M. Ishida, C. Yutani, K. Miyatake, and S. Kitamura A novel application of myocardial contrast echocardiography to evaluate angiogenesis by autologous bone marrow cell transplantation in chronic ischemic pig model J. Am. Coll. Cardiol., April 7, 2004; 43(7): 1299 - 1305. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. M. Yau, G. Li, R. D. Weisel, A. Reheman, Z.-Q. Jia, D. A. G. Mickle, and R.-K. Li Vascular endothelial growth factor transgene expression in cell-transplanted hearts J. Thorac. Cardiovasc. Surg., April 1, 2004; 127(4): 1180 - 1187. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. C. Hughes, S. S. Biswas, B. Yin, R. E. Coleman, T. R. DeGrado, C. K Landolfo, J. E. Lowe, B. H. Annex, and K. P. Landolfo Therapeutic angiogenesis in chronically ischemic porcine myocardium: comparative effects of bFGF and VEGF Ann. Thorac. Surg., March 1, 2004; 77(3): 812 - 818. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. C. Chachques, C. Acar, J. Herreros, J. C. Trainini, F. Prosper, N. D'Attellis, J.-N. Fabiani, and A. F. Carpentier Cellular cardiomyoplasty: clinical application Ann. Thorac. Surg., March 1, 2004; 77(3): 1121 - 1130. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Fujii, T. M. Yau, R. D. Weisel, N. Ohno, D. A. G. Mickle, N. Shiono, T. Ozawa, K. Matsubayashi, and R.-K. Li Cell transplantation to prevent heart failure: a comparison of cell types Ann. Thorac. Surg., December 1, 2003; 76(6): 2062 - 2070. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. H.L. Tang, P. W.M. Fedak, T. M. Yau, R. D. Weisel, A. Kulik, D. A.G. Mickle, and R.-K. Li Cell transplantation to improve ventricular function in the failing heart Eur. J. Cardiothorac. Surg., June 1, 2003; 23(6): 907 - 916. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Menasche Cell transplantation in myocardium Ann. Thorac. Surg., June 1, 2003; 75(90060): S20 - 28. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. D. Dowell, M. Rubart, K. B.S. Pasumarthi, M. H. Soonpaa, and L. J. Field Myocyte and myogenic stem cell transplantation in the heart Cardiovasc Res, May 1, 2003; 58(2): 336 - 350. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. C.-J. Chiu Therapeutic cardiac angiogenesis and myogenesis: The promises and challenges on a new frontier J. Thorac. Cardiovasc. Surg., March 1, 2003; 125(90030): S55 - 56. [Full Text] [PDF]
|
|
|
|
|
|
O. O. Al-Radi, V. Rao, R.-k. Li, T. Yau, and R. D. Weisel Cardiac cell transplantation: closer to bedside Ann. Thorac. Surg., February 1, 2003; 75(2): S674 - 677. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. M. Yau, S. Tomita, R. D. Weisel, Z.-Q. Jia, L. C. Tumiati, D. A.G. Mickle, and R.-K. Li Beneficial effect of autologous cell transplantation on infarcted heart function: comparison between bone marrow stromal cells and heart cells Ann. Thorac. Surg., January 1, 2003; 75(1): 169 - 177. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Ruel, R. A. Kelly, and F. W. Sellke Therapeutic Angiogenesis, Transmyocardial Laser Revascularization, and Cell Therapy Card. Surg. Adult, January 1, 2003; 2(2003): 715 - 750. [Full Text]
|
|
|
|
|
|
S. Tomita, D. A. G. Mickle, R. D. Weisel, Z.-Q. Jia, L. C. Tumiati, Y. Allidina, P. Liu, and R.-K. Li Improved heart function with myogenesis and angiogenesis after autologous porcine bone marrow stromal cell transplantation J. Thorac. Cardiovasc. Surg., June 1, 2002; 123(6): 1132 - 1140. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. C.-J. Chiu Therapeutic cardiac angiogenesis and myogenesis: The promises and challenges on a new frontier J. Thorac. Cardiovasc. Surg., November 1, 2001; 122(5): 851 - 852. [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| ANN THORAC SURG | ASIAN CARDIOVASC THORAC ANN | EUR J CARDIOTHORAC SURG |
| J THORAC CARDIOVASC SURG | ICVTS | ALL CTSNet JOURNALS |
