JTCS Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Personal Folders
Right arrow Download to citation manager
Right arrow Author home page(s):
Matthias Karck
Steven F. Bolling
Axel Haverich
Right arrow Permission Requests
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Karck, M.
Right arrow Articles by Haverich, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Karck, M.
Right arrow Articles by Haverich, A.
Related Collections
Right arrow Cardiac - physiology
Right arrow Myocardial protection

J Thorac Cardiovasc Surg 2001;122:986-992
© 2001 The American Association for Thoracic Surgery

Cardiopulmonary Support and Physiology (CSP)

Myocardial protection by ischemic preconditioning and {delta}-opioid receptor activation in the isolated working rat heart

Matthias Karck, MDa, Satonori Tanaka, MDa, Steven F. Bolling, MDb, Andre Simon, MDa, Tsung-Ping Su, PhDc, Peter R. Oeltgen, PhDd, Axel Haverich, MDa

From the Department of Thoracic and Cardiovascular Surgery,a Hannover Medical School, Hannover, Germany; Section of Cardiac Surgery,b University of Michigan Hospitals, Ann Arbor, Mich; National Institute on Drug Abuse,c Addiction Research Center, Baltimore, Md; and the Department of Pathology,d University of Kentucky College of Medicine, Lexington, Ky.

This study was supported by a grant from the Deutsche Forschungsgemeinschaft (Ka 1169/1-2).

Received for publication Dec 21, 2000. Revisions requested Jan 25, 2001; revisions received April 19, 2001. Accepted for publication April 24, 2001. Address for reprints: Matthias Karck, MD, Department of Thoracic and Cardiovascular Surgery, Hannover Medical School, 30623 Hannover, Germany.

Abstract

Objective: {delta}-Opioid receptors are involved in the cardioprotective effect of ischemic preconditioning. This study was designed (1) to assess the protective capacities of ischemic preconditioning and the synthetic {delta}-opioid receptor agonist D-Ala2-D-Leu5 enkephalin (DADLE) in a functionally oriented experimental model of ischemia and reperfusion and (2) to assess whether the effects of both protective measures are similarly blocked by naloxone, a nonspecific {delta}-opioid receptor antagonist.
Methods: Sixty-four isolated working rat hearts were subjected to 45 minutes of hypothermic ischemia at 30°C followed by 25 minutes of normothermic reperfusion. Rats were pretreated with DADLE (1 mg/kg body weight intravenously), naloxone (3 mg/kg body weight intravenously), or a combination thereof within 60 minutes before onset of isolated heart perfusion. During the preischemic perfusion period, 8 hearts per group were preconditioned by one cycle of 5 minutes of normothermic global ischemia and subsequent reperfusion whereas another 8 served as nonpreconditioned controls. The postischemic functional recovery of hearts and their creatine kinase leakage were determined.
Results: Pretreatment with DADLE and ischemic preconditioning improved the postischemic recovery of aortic flow when compared with nonpreconditioning (57.7% ± 4.0% and 60.8% ± 4.3% vs 40.0% ± 4.2% of preischemic baseline value, P < .001). Combined pretreatment with DADLE before ischemic preconditioning afforded additional aortic flow recovery compared with pretreatment with DADLE alone (68.6% ± 3.3% vs 57.7% ± 4.0% of preischemic baseline value; P = .038). With combined pretreatment, early postischemic creatine kinase release was lower than control in hearts without pretreatment (0.48 ± 0.11 vs 0.80 ± 0.12 IU/5 minutes per heart; P = .001). Naloxone abolished the beneficial functional effects of pretreatment with DADLE and ischemic preconditioning.
Conclusions: Pharmacologic activation of {delta}-opioid receptors affords improvement of functional protection in isolated working rat hearts similar to that conferred by classic ischemic preconditioning. The combination of both pretreatments reduces ischemic cellular damage and further adds to postischemic functional recovery. These changes are reversed by naloxone, an observation providing evidence that ischemic preconditioning involves signaling through opioid receptors.




This article has been cited by other articles:


Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
A. J. Zatta, H. Kin, D. Yoshishige, R. Jiang, N. Wang, J. G. Reeves, J. Mykytenko, R. A. Guyton, Z.-Q. Zhao, J. L. Caffrey, et al.
Evidence that cardioprotection by postconditioning involves preservation of myocardial opioid content and selective opioid receptor activation
Am J Physiol Heart Circ Physiol, March 1, 2008; 294(3): H1444 - H1451.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
S. Pepe, O. W.V van den Brink, E. G Lakatta, and R.-P. Xiao
Cross-talk of opioid peptide receptor and {beta}-adrenergic receptor signalling in the heart
Cardiovasc Res, August 15, 2004; 63(3): 414 - 422.
[Abstract] [Full Text] [PDF]

Home page
 Eur. J. Cardiothorac. Surg.Home page
F. Nicolini, C. Beghi, C. Muscari, A. Agostinelli, A. M. Budillon, I. Spaggiari, and T. Gherli
Myocardial protection in adult cardiac surgery: current options and future challenges
Eur. J. Cardiothorac. Surg., December 1, 2003; 24(6): 986 - 993.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ANN THORAC SURG ASIAN CARDIOVASC THORAC ANN EUR J CARDIOTHORAC SURG
J THORAC CARDIOVASC SURG ICVTS ALL CTSNet JOURNALS
Copyright © 2001 by The American Association for Thoracic Surgery.