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J Thorac Cardiovasc Surg 2002;123:98-103
© 2002 The American Association for Thoracic Surgery
Cardiothoracic Transplantation |
From the Division of Thoracic and Cardiovascular Surgerya and Department of Respiratory Medicine,b Hannover Medical School, Hannover, Germany.
Received for publication Feb 12, 2001. Revisions requested April 17, 2001; revisions received July 20, 2001. Accepted for publication July 25, 2001. Address for reprints: Martin Strüber, MD, Division of Thoracic and Cardiovascular Surgery, Hannover Medical School, Carl Neuberg Str 1, 30623 Hannover, Germany (E-mail: strueber{at}thg.mh-hannover.de).
Objective: Surfactant function was shown to be impaired in clinical and experimental lung transplantation. This study was designed to define the impact of retrograde flush perfusion on graft and surfactant function after an extended period of ischemia.
Methods: Left lung transplantation was performed after 24 hours of graft ischemia in 12 pigs. In half of the grafts antegrade cold flush perfusion (Perfadex) was used for preservation. In the second group grafts were flushed in a retrograde fashion via the left atrium. Graft function was monitored for 7 hours after transplantation. Before transplantation (basal) and after 2 hours of reperfusion, bronchoalveolar lavage fluid was obtained. Minimal surface tension of bronchoalveolar lavage fluid was determined and the ratio of small and large surfactant aggregates was calculated. Lung water content was analyzed online in the reperfusion period.
Results: Right-sided heart failure developed in 2 animals of group 1 (antegrade perfusion) within 2 and 4.5 hours of reperfusion, respectively. All other pigs survived the observation period. PO2/FIO2 (P = .001) and dynamic lung compliance (P = .001) were superior in retrogradely flushed grafts. A comparable increase of minimal surface tension was found after reperfusion in both groups. Small/large surfactant aggregate ratio after reperfusion (P = .03), as well as extravascular lung water content, was higher in the antegrade perfusion group.
Conclusion: Retrograde flush perfusion for 24-hour lung preservation with low-potassium dextran (Perfadex) solution led to better initial graft function than the standard antegrade perfusion technique. A moderate impairment of surfactant function was found in both groups, which was more pronounced in the antegrade perfusion group.
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