HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Personal Folders Download to citation manager Author home page(s): Magdi H. Yacoub Permission Requests Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Suzuki, K. Articles by Yacoub, M. H. Search for Related Content PubMed PubMed Citation Articles by Suzuki, K. Articles by Yacoub, M. H. Related Collections Molecular biology

J Thorac Cardiovasc Surg 2002;123:984-992
© 2002 The American Association for Thoracic Surgery

Evolving Technology (ET)

Single fibers of skeletal muscle as a novel graft for cell transplantation to the heart

From the Department of Cardiothoracic Surgery, Heart Science Centre, Harefield Hospital,^a and Muscle Cell Biology Group, Hammersmith Hospital, Imperial College Faculty of Medicine,^b London, United Kingdom.

This study was supported by the British Heart Foundation and the Harefield Research Foundation. Ken Suzuki is supported by the research fellowship from the Uehara Memorial Foundation.

Received for publication April 30, 2001. Revisions requested July 9, 2001; revisions received Oct 24, 2001. Accepted for publication Nov 7, 2001. Address for reprints: Professor Sir Magdi H. Yacoub, Department of Cardiothoracic Surgery, Harefield Hospital, Hill End Rd, Harefield, Middlesex, UB9 6JH, United Kingdom (E-mail: k.suzuki{at}ic.ac.uk).

Objective: Skeletal myoblast transplantation is a promising alternative to treat heart failure. A single fiber, the minimal functional unit of skeletal muscle, retains skeletal myoblasts beneath the basal lamina. When surrounding muscle is injured, myoblasts migrate from the fiber into the damaged area to regenerate muscle. We hypothesized that such isolated fibers could be used as an efficient vehicle to deliver myoblasts into damaged myocardium, resulting in improved cardiac function.
Methods: Living single fibers of rat skeletal muscle were isolated, and their behavior was characterized in vitro. Single fibers were injected into the myocardium (at 4 sites, each receiving a single fiber) of rats in 2 models of heart failure induced either by means of doxorubicin administration or left coronary artery occlusion.
Results: Skeletal myoblasts dissociated from an isolated single fiber, proliferated, and differentiated into multinucleated myotubes in vitro. Within 3 days after grafting in vivo, original fibers provided putative myoblasts and disappeared. At 4 weeks, discrete loci consisting of several multinucleated myotubes were observed. Furthermore, single-fiber transplantation significantly improved cardiac function compared with the control treatment in either doxorubicin-treated hearts (maximum dP/dt, 4013.9 ± 96.1 vs 3603.1 ± 102.3 mm Hg/s; minimum dP/dt, -2313.7 ± 75.1 vs -2057.1 ± 52.4 mm Hg/s) or ischemic hearts (maximum dP/dt, 3905.6 ± 103.0 vs 3572.6 ± 109.7 mm Hg/s; minimum dP/dt, -2336.1 ± 69.7 vs -2106.4 ± 74.2 mm Hg/s).
Conclusion: Single-fiber transplantation acts as a vehicle for delivering putative skeletal myoblasts that appear to differentiate into myotubes within the myocardium. This was associated with improved function of failing hearts, suggesting its efficacy as a novel graft for cellular cardiomyoplasty.

This article has been cited by other articles:

				I. A. Memon, Y. Sawa, N. Fukushima, G. Matsumiya, S. Miyagawa, S. Taketani, S. K. Sakakida, H. Kondoh, A. N. Aleshin, T. Shimizu, et al. Repair of impaired myocardium by means of implantation of engineered autologous myoblast sheets J. Thorac. Cardiovasc. Surg., November 1, 2005; 130(5): 1333 - 1341. [Abstract] [Full Text] [PDF]

				I. A. Memon, Y. Sawa, S. Miyagawa, S. Taketani, and H. Matsuda Combined autologous cellular cardiomyoplasty with skeletal myoblasts and bone marrow cells in canine hearts for ischemic cardiomyopathy J. Thorac. Cardiovasc. Surg., September 1, 2005; 130(3): 646 - 653. [Abstract] [Full Text] [PDF]

				K. Suzuki, B. Murtuza, J. R. Beauchamp, N. J. Brand, P. J. R. Barton, A. Varela-Carver, S. Fukushima, S. R. Coppen, T. A. Partridge, and M. H. Yacoub Role of Interleukin-1{beta} in Acute Inflammation and Graft Death After Cell Transplantation to the Heart Circulation, September 14, 2004; 110(11_suppl_1): II-219 - II-224. [Abstract] [Full Text] [PDF]

				K. Suzuki, B. Murtuza, S. Fukushima, R. T. Smolenski, A. Varela-Carver, S. R. Coppen, and M. H. Yacoub Targeted Cell Delivery Into Infarcted Rat Hearts by Retrograde Intracoronary Infusion: Distribution, Dynamics, and Influence on Cardiac Function Circulation, September 14, 2004; 110(11_suppl_1): II-225 - II-230. [Abstract] [Full Text] [PDF]

				N. Borenstein, P. Bruneval, M. Hekmati, C. Bovin;, L. Behr, C. Pinset, F. Laborde, and D. Montarras Noncultured, Autologous, Skeletal Muscle Cells Can Successfully Engraft Into Ovine Myocardium Circulation, June 24, 2003; 107(24): 3088 - 3092. [Abstract] [Full Text] [PDF]

				E. K. W, H. K. Haider, and P. K. Law Single fiber skeletal muscle transplantation or purified myoblast engraftment? J. Thorac. Cardiovasc. Surg., May 1, 2003; 125(5): 1181 - 1182. [Full Text] [PDF]

				J. D. Dowell, M. Rubart, K. B.S. Pasumarthi, M. H. Soonpaa, and L. J. Field Myocyte and myogenic stem cell transplantation in the heart Cardiovasc Res, May 1, 2003; 58(2): 336 - 350. [Abstract] [Full Text] [PDF]

				F. J. Schoen and R. F. Padera Jr. Cardiac Surgical Pathology Card. Surg. Adult, January 1, 2003; 2(2003): 119 - 185. [Full Text]