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J Thorac Cardiovasc Surg 2003;126:208-215
© 2003 The American Association for Thoracic Surgery

Cardiothoracic transplantation

Impaired endothelium-derived hyperpolarizing factor–mediated relaxation in porcine pulmonary microarteries after cold storage with Euro-Collins and University of Wisconsin solutions

^a Department of Surgery, The Chinese University of Hong Kong, Hong Kong SAR, China
^b Starr Academic Center, Providence Heart Institute, Oregon Health and Science University, Portland, Ore, USA

Presented as an abstract at the 74nd Scientific Sessions, American Heart Association, Anaheim, Calif, Nov 12-15, 2001.

Received for publication June 14, 2002. Received for publication September 11, 2002; revisions received September 14, 2002; accepted for publication September 23, 2002.

^* Address for reprint requests: Professor Guo-Wei He, MD, PhD, Department of Surgery, The Chinese University of Hong Kong, Block B, 5A, Prince of Wales Hospital, Shatin, N.T., Hong Kong SAR, China
gwhe{at}cuhk.edu.hk

BACKGROUND: Endothelium plays an important role in mediating the function of transplanted organs. The widely used University of Wisconsin solution impairs the endothelium-derived hyperpolarizing factor–mediated relaxation in coronary arteries, but little is known about effects of lung preservation on endothelium-derived hyperpolarizing factor–mediated endothelial function. This study examined the effect of organ preservation solutions on the endothelium-derived hyperpolarizing factor–mediated relaxation in the pulmonary microarteries (diameter 200 to 450 µm).

METHODS: Two segments (1 as control) from the same microartery were allocated in 2 chambers of a myograph. After incubation with hyperkalemia (potassium 115 mmol/L), University of Wisconsin, or Euro-Collins solution (at 4°C for 4 hours), the endothelium-derived hyperpolarizing factor–mediated relaxation was induced by bradykinin (-10 to -6.5 log M, n = 8) or calcium ionophore (A₂₃₁₈₇, -9 to -5.5 log M, n = 7) in U₄₆₆₁₉ (-7.5 log M) precontracted rings in the presence of indomethacin (7 µmol/L), N^G-nitro-L-arginine (300 µmol/L), and oxyhemoglobin (20 µmol/L).

RESULTS: Exposure to hyperkalemia and storage with Euro-Collins or University of Wisconsin solution significantly decreased the relaxation to bradykinin (51.9 ± 8.4% vs 60.3 ± 6.1%, P = .02 or 49.3 ± 7.3% vs 65.2 ± 3.5%, P = .04) or A₂₃₁₈₇ (12.5 ± 0.02% vs 33.8 ± 0.07%, P = .02 or 13.2 ± 0.03% vs 31.0 ± 0.05%, P = .03%).

CONCLUSIONS: Endothelium-derived hyperpolarizing factor plays an important role in porcine pulmonary microarteries, and the endothelium-derived hyperpolarizing factor–mediated relaxation is impaired when the lung is preserved with University of Wisconsin or Euro-Collins solution. This impairment may affect the lung function during the reperfusion period after lung transplantation.

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