HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Personal Folders Download to citation manager Permission Requests Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kojima, K. Articles by Vacanti, C. A. Search for Related Content PubMed PubMed Citation Articles by Kojima, K. Articles by Vacanti, C. A. Related Collections Trachea and bronchi

J Thorac Cardiovasc Surg 2004;128:147-153
© 2004 The American Association for Thoracic Surgery

General thoracic surgery

Tissue-engineered trachea from sheep marrow stromal cells with transforming growth factor ß2 released from biodegradable microspheres in a nude rat recipient

^a Laboratory for Tissue Engineering and Regenerative Medicine, Brigham & Women's Hospital, Harvard Medical School, Worcester, Mass, USA
^b Center for Tissue Engineering, University of Massachusetts Medical School, Boston, Mass, USA
^c Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan

Read at the Eighty-third Annual Meeting of The American Association for Thoracic Surgery, Boston, Mass, May 4-7, 2003.

Received for publication May 11, 2003; revisions received February 4, 2004; accepted for publication February 26, 2004.

^* Address for reprints: Koji Kojima, MD, PhD, Laboratory for Tissue Engineering and Regenerative Medicine, Department of Anesthesiology, Brigham and Women's Hospital, 75 Francis St, Thorn 1334, Boston, MA 02115, USA
kojima{at}zeus.bwh.harvard.edu

OBJECTIVE: The purpose of this study was to evaluate the feasibility of using autologous sheep marrow stromal cells cultured onto polyglycolic acid mesh to develop helical engineered cartilage equivalents for a functional tracheal replacement. We also explored the potential benefit of local delivery of transforming growth factor ß2 with biodegradable gelatin microspheres.

METHODS: Bone marrow was obtained by iliac crest aspiration from 6-month-old sheep and cultured in monolayer for 2 weeks. At confluence, the cells were seeded onto nonwoven polyglycolic acid fiber mesh and cultured in vitro with transforming growth factor ß2 and insulin-like growth factor 1 for 1 week. Cell-polymer constructs were wrapped around a silicone helical template. Constructs were then coated with microspheres incorporating 0.5 µg transforming growth factor ß2. The cell-polymer-microsphere structures were then implanted into a nude rat. On removal, glycosaminoglycan content and hydroxyproline were analyzed in both native and tissue-engineered trachea. Histologic sections of both native and tissue-engineered trachea were stained with hematoxylin and eosin, safranin-O, and a monoclonal anti–type II collagen antibody.

RESULTS: Cell-polymer constructs with transforming growth factor ß2 microspheres formed stiff cartilage de novo in the shape of a helix after 6 weeks. Control constructs lacking transforming growth factor ß2 microspheres appeared to be much stiffer than typical cartilage, with an apparently mineralized matrix. Tissue-engineered trachea was similar to normal trachea. Histologic data showed the presence of mature cartilage. Glycosaminoglycan and hydroxyproline contents were also similar to native cartilage levels.

CONCLUSIONS: This study demonstrates the feasibility of engineering tracheas with sheep marrow stromal cells as a cell source. Engineering the tracheal equivalents with supplemental transforming growth factor ß2 seemed to have a positive effect on retaining a cartilaginous phenotype in the newly forming tissue.

This article has been cited by other articles:

				T. Nakamura, T. Sato, M. Araki, S. Ichihara, A. Nakada, M. Yoshitani, S.-i. Itoi, M. Yamashita, S.-i. Kanemaru, K. Omori, et al. In situ tissue engineering for tracheal reconstruction using a luminar remodeling type of artificial trachea J. Thorac. Cardiovasc. Surg., October 1, 2009; 138(4): 811 - 819. [Abstract] [Full Text] [PDF]

				D. Fabre, S. Singhal, V. De Montpreville, B. Decante, S. Mussot, O. Chataigner, O. Mercier, F. Kolb, P. G. Dartevelle, and E. Fadel Composite cervical skin and cartilage flap provides a novel large airway substitute after long-segment tracheal resection J. Thorac. Cardiovasc. Surg., July 1, 2009; 138(1): 32 - 39. [Abstract] [Full Text] [PDF]

				T. Sato, H. Tao, M. Araki, H. Ueda, K. Omori, and T. Nakamura Replacement of the Left Main Bronchus With a Tissue-Engineered Prosthesis in a Canine Model Ann. Thorac. Surg., August 1, 2008; 86(2): 422 - 428. [Abstract] [Full Text] [PDF]

				D. J. Weiss, J. K. Kolls, L. A. Ortiz, A. Panoskaltsis-Mortari, and D. J. Prockop Stem Cells and Cell Therapies in Lung Biology and Lung Diseases Proceedings of the ATS, July 15, 2008; 5(5): 637 - 667. [Full Text] [PDF]