HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Personal Folders Download to citation manager Author home page(s): Marc Ruel Derek Gunning Fraser D. Rubens Thierry G. Mesana Permission Requests Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Ruel, M. Articles by Mesana, T. G. Search for Related Content PubMed PubMed Citation Articles by Ruel, M. Articles by Mesana, T. G. Related Collections Cardiac - physiology Coronary disease Extracorporeal circulation Molecular biology

J Thorac Cardiovasc Surg 2005;130:633-639
© 2005 The American Association for Thoracic Surgery

Cardiopulmonary Support and Physiology

Effects of off-pump versus on-pump coronary artery bypass grafting on function and viability of circulating endothelial progenitor cells

Division of Cardiac Surgery, University of Ottawa, Ottawa, Ontario, Canada

Received for publication October 29, 2004; revisions received December 16, 2004; accepted for publication January 10, 2005.

^_* Address for reprints: Marc Ruel, MD, MPH, University of Ottawa Heart Institute, 40 Ruskin St, Ste 3403, Ottawa, Ontario, Canada K1Y 4W7 (Email: mruel{at}ottawaheart.ca).

OBJECTIVE: Off-pump coronary artery bypass grafting may result in fewer myocardial and vascular complications than on-pump. Although differences in aortic manipulations likely play a role, the systemic responses of endothelial progenitor cells to both types of operations have not been examined. We sought to examine endothelial progenitor cell characteristics after off-pump versus on-pump coronary artery bypass grafting.

METHODS: Twenty patients undergoing off-pump or on-pump coronary artery bypass grafting were prospectively enrolled and had endothelial progenitor cells isolated and cultured from their peripheral blood before and 24 hours after surgery. Endothelial progenitor cells were identified by fluorescent dual lectin/low-density lipoprotein binding. Their number, phenotype characteristics, proliferation, migratory function, and viability were determined in a blinded fashion.

RESULTS: Patient characteristics and numbers of grafts were equivalent. Endothelial progenitor cells had similar phenotypes between groups before and after surgery. Off-pump and on-pump coronary artery bypass grafting resulted in similar increases in endothelial progenitor cell numbers and showed equivalent proliferation activity. However, endothelial progenitor cell migratory function was higher in off-pump patients (25.3 ± 5.0 vs 5.0 ± 1.0 cells per high-powered field for off-pump vs on-pump coronary artery bypass grafting, respectively; P = .04). Postoperative endothelial progenitor cell viability adjusted for preoperative baseline was also higher after off-pump than on-pump coronary artery bypass grafting by 72.4% ± 14.6% (P = .01). Endothelial progenitor cells of on-pump patients were less viable after surgery than before surgery, whereas the reverse was observed in off-pump patients.

CONCLUSIONS: Both on-pump and off-pump coronary artery bypass grafting elicit mobilization of endothelial progenitor cells into the peripheral blood. On-pump coronary artery bypass grafting, however, impairs the migratory function and viability of these vascular repair cells, which are conversely preserved after off-pump surgery. Further work is necessary to determine whether the function and viability of endothelial progenitor cells correlate with vascular outcomes and whether their therapeutic modulation may one day benefit coronary artery bypass grafting patients.

This article has been cited by other articles:

				E. J. Suuronen, P. Zhang, D. Kuraitis, X. Cao, A. Melhuish, D. McKee, F. Li, T. G. Mesana, J. P. Veinot, and M. Ruel An acellular matrix-bound ligand enhances the mobilization, recruitment and therapeutic effects of circulating progenitor cells in a hindlimb ischemia model FASEB J, May 1, 2009; 23(5): 1447 - 1458. [Abstract] [Full Text] [PDF]

				Y. Zhang, S. Thorn, J. N. DaSilva, M. Lamoureux, R. A. deKemp, R. S. Beanlands, M. Ruel, and E. J. Suuronen Collagen-Based Matrices Improve the Delivery of Transplanted Circulating Progenitor Cells: Development and Demonstration by Ex Vivo Radionuclide Cell Labeling and In Vivo Tracking With Positron-Emission Tomography Circ Cardiovasc Imaging, November 1, 2008; 1(3): 197 - 204. [Abstract] [Full Text] [PDF]

				W Wojakowski, M Kucia, M Kazmierski, M Z Ratajczak, and M Tendera Circulating progenitor cells in stable coronary heart disease and acute coronary syndromes: relevant reparatory mechanism? Heart, January 1, 2008; 94(1): 27 - 33. [Abstract] [Full Text] [PDF]

				E. J. Suuronen, J. Price, J. P. Veinot, K. Ascah, V. Kapila, X.-W. Guo, S. Wong, T. G. Mesana, and M. Ruel Comparative effects of mesenchymal progenitor cells, endothelial progenitor cells, or their combination on myocardial infarct regeneration and cardiac function. J. Thorac. Cardiovasc. Surg., November 1, 2007; 134(5): 1249 - 1258. [Abstract] [Full Text] [PDF]

				N. Roberts, Q. Xiao, G. Weir, Q. Xu, and M. Jahangiri Endothelial Progenitor Cells are Mobilized After Cardiac Surgery Ann. Thorac. Surg., February 1, 2007; 83(2): 598 - 605. [Abstract] [Full Text] [PDF]

				S. Mieno, B. Ramlawi, M. Boodhwani, R. T. Clements, K. Minamimura, T. Maki, S.-H. Xu, C. Bianchi, J. Li, and F. W. Sellke Role of Stromal-Derived Factor-1{alpha} in the Induction of Circulating CD34+CXCR4+ Progenitor Cells After Cardiac Surgery. Circulation, July 4, 2006; 114(1 Suppl): 186 - 192. [Abstract] [Full Text] [PDF]

				E. J. Suuronen, J. P. Veinot, S. Wong, V. Kapila, J. Price, M. Griffith, T. G. Mesana, and M. Ruel Tissue-Engineered Injectable Collagen-Based Matrices for Improved Cell Delivery and Vascularization of Ischemic Tissue Using CD133+ Progenitors Expanded From the Peripheral Blood Circulation, July 4, 2006; 114(1_suppl): I-138 - I-144. [Abstract] [Full Text] [PDF]

				E. J. Suuronen, S. Wong, V. Kapila, G. Waghray, S. C. Whitman, T. G. Mesana, and M. Ruel Generation of CD133+ cells from CD133- peripheral blood mononuclear cells and their properties Cardiovasc Res, April 1, 2006; 70(1): 126 - 135. [Abstract] [Full Text] [PDF]