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Mitsuhiro Kawata
Shinichi Takamoto
Tetsuro Morota
Minoru Ono
Noboru Motomura
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Yoshihiro Suematsu
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J Thorac Cardiovasc Surg 2006;131:1331-1337
© 2006 The American Association for Thoracic Surgery

Cardiopulmonary Support and Physiology

Erythropoietin protects the central nervous system during prolonged hypothermic circulatory arrest: An experimental study in a canine model

Mitsuhiro Kawata, MD * , Shinichi Takamoto, MD, PhD, Kazuo Kitahori, MD, PhD, Hiroyuki Tsukihara, MD, Tetsuro Morota, MD, PhD, Minoru Ono, MD, PhD, Noboru Motomura, MD, PhD, Arata Murakami, MD, PhD, Yoshihiro Suematsu, MD, PhD

Department of Cardiothoracic Surgery, Graduate School of Medicine, University of Tokyo, Tokyo, Japan.

Received for publication May 27, 2005; revisions received September 29, 2005; accepted for publication October 3, 2005.

* Address for reprints: Mitsuhiro Kawata, MD, Department of Cardiothoracic Surgery, Graduate School of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-8655, Japan. (Email: mkawata-ths{at}umin.ac.jp).

OBJECTIVE: Current data suggest that erythropoietin protects the brain and the spinal cord from ischemic and traumatic injury. In this study, we determined whether erythropoietin protects the central nervous system during prolonged hypothermic circulatory arrest in an experimental canine model.

METHODS: Ten adult beagle dogs were randomly and intravenously injected with either 5000 U/kg recombinant human erythropoietin or normal saline. Each dog was then subjected to a cardiopulmonary bypass and 120 minutes of deep hypothermic circulatory arrest (18°C). The level of tau proteins in the cerebrospinal fluid, a modified marker of neurologic deficit in dogs, and the histopathologic characteristics of the brains and spinal cords were then examined.

RESULTS: The level of tau proteins was significantly lower in the erythropoietin-treated group than in the untreated group at 6 hours (20 ± 12 vs 144 ± 54 pg/mL; P = .036) and 12 hours (64 ± 35 vs 478 ± 103 pg/mL; P = .01) after the operation. The total Neurologic Deficit Score was 59 ± 31 (0, normal; 500, brain death) in the erythropoietin-treated group, compared with 376 ± 30 in the untreated group (P = .0117). Histopathologic examination revealed that ischemic neuronal changes and apoptosis in the hippocampus CA1 were significantly lower in the erythropoietin-treated group (P < .01 and P = .028, respectively).

CONCLUSIONS: This study showed that erythropoietin protected the central nervous system during prolonged hypothermic circulatory arrest, partly by preventing both necrosis (ischemic neuronal changes) and apoptosis.

Abbreviations and Acronyms CPB = cardiopulmonary bypass; CSF = cerebrospinal fluid; EPO = erythropoietin; HDS = Histopathologic Damage Score; NDS = Neurologic Deficit Score; TUNEL = deoxyuridine-5'-triphosphate biotin nick end labeling


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J. Thorac. Cardiovasc. Surg. 2006 131: 1226. [Extract] [Full Text] [PDF]





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