JTCS Tips for Better Browsing
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to Personal Folders
Right arrow Download to citation manager
Right arrow Author home page(s):
Guido J. Van Nooten
Kishan Narine
Yves Van Belleghem
Right arrow Permission Requests
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Van Nooten, G. J.
Right arrow Articles by De Somer, F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Van Nooten, G. J.
Right arrow Articles by De Somer, F.

J Thorac Cardiovasc Surg 2007;134:415-423
© 2007 The American Association for Thoracic Surgery

Evolving Technology

Autologous glue: Part of the sticky mystery unraveled

Guido J. Van Nooten, MD, PhDa,*, Pamela Somers, BSca, Ramses Forsyth, MDb, Kishan Narine, MD, PhDa, Yves Van Belleghem, MD, BSca, Stefaan Jacobs, MDa, Filip De Somer, PhDa

a Heart Center, University Hospital Ghent, Ghent, Belgium
b Department of Pathology, University Hospital Ghent, Ghent, Belgium.

Received for publication January 25, 2007; revisions received March 16, 2007; accepted for publication April 2, 2007.

* Address for reprints: Guido J. Van Nooten, MD, PhD, Cardiac Surgery Department 5K12, University Hospital Ghent, Belgium, De Pintelaan 185, 9000 Ghent, Belgium. (Email: guido.vannooten{at}UGent.be).

Objective: The aim of the study was to evaluate the safety and efficacy of an autologous surgical tissue adhesive.

Methods: Autologous glue was made out of canine concentrated plasma proteins mixed with 7.5% glutaraldehyde. Tensile strength and cytotoxicity of the autologous glue were tested. In a dog model, 8 transectioned iliac arteries were reanastomosed by using the animal’s glue as the sole fixation method. After 120 days, all animals were angiographically controlled for patency and killed for histologic and immunohistochemical examination of the anastomosis.

Results: The autologous glue showed sufficient tensile strength (557 ± 135 N/mm2). The elasticity of the glue is influenced by variations of concentrations in both proteins and glutaraldehyde. Glutaraldehyde remained cytotoxic, even at low concentrations of 2.5%. All operative procedures were successful. Angiographs performed before animal death showed all but 1 vessel to be patent and showed manifest compression signs in 3 anastomoses. Histological examination revealed only a foreign-body reaction adjacent to the surface of the glue. The autologous glue does not trigger any immune response on immunochemistry. Because fibroblastic neo-endothelial lining was near to normal, potential glutaraldehyde leaching does not seem too harmful for the vascular juncture in the dog model.

Conclusions: Autologous glutaraldehyde glue has been used successfully as a vascular adhesive. In contrast to our previous studies with heterologous glue, we did not find a fierce acute inflammatory reaction indicating immune triggering. Nevertheless, glutaraldehyde remains a cytotoxic cross-linker. It is yet not known whether autologous glutaraldehyde glue can be used safely in clinical practice.

Abbreviations and Acronyms GRF = gelatin-resorcinol-formaldehyde; MTS = [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt]; PBS = phosphate-buffered saline; TIMI = Thrombolysis In Myocardial Infarction






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ANN THORAC SURG ASIAN CARDIOVASC THORAC ANN EUR J CARDIOTHORAC SURG
J THORAC CARDIOVASC SURG ICVTS ALL CTSNet JOURNALS
Copyright © 2007 by The American Association for Thoracic Surgery.