JTCS
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Add to Personal Folders
Right arrow Download to citation manager
Right arrow Permission Requests
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zhou, J.
Right arrow Articles by Lee, T. D.G.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Zhou, J.
Right arrow Articles by Lee, T. D.G.
Related Collections
Right arrow Cardiac - other
Right arrow Extracorporeal circulation

J Thorac Cardiovasc Surg 2008;135:777-783
© 2008 The American Association for Thoracic Surgery

Cardiopulmonary Support and Physiology

Reduction in postsurgical adhesion formation after cardiac surgery in a rabbit model using N,O-carboxymethyl chitosan to block cell adherence

Juan Zhou, MD, PhDa, Robert S. Liwski, MD, PhDb, Clive Elson, PhDc, Timothy D.G. Lee, PhDa,b,*

a Department of Surgery, Faculty of Medicine, Dalhousie University, Halifax, Nova Scotia
b Department of Pathology, Faculty of Medicine, Dalhousie University, Halifax, Nova Scotia
c Department of Chemistry, Saint Mary's University, Halifax, Nova Scotia

Received for publication January 18, 2007; revisions received August 23, 2007; accepted for publication September 19, 2007.

* Address for reprints: Tim Lee, PhD, Sir Charles Tupper Building, 5850 College Street, Dalhousie University, Halifax, NS, Canada, B3H 4H7. (Email: tim.lee{at}dal.ca).

Objective: The study objectives were to (1) assess the efficacy of N,O-carboxymethyl chitosan on postsurgical adhesion formation after cardiac surgery using a rabbit cardiac injury model and (2) explore the mechanism of action of N,O-carboxymethyl chitosan in the prevention of postsurgical adhesions using in vitro experimentation.

Methods: In the rabbit cardiac injury model, cardiac injury was generated by abrading the anterior surface of the heart with gauze and desiccated with oxygen. The rabbits were then either treated with N,O-carboxymethyl chitosan gel and solution on the injured surface or not treated. Fourteen days or 3 months after surgery, the severity and area of adhesion between the heart and sternum were evaluated. In the in vitro adherence assay, murine fibroblasts and macrophages were labeled with 3H-thymidine and added to sterile tissue culture plates that had been precoated with N,O-carboxymethyl chitosan solution, culture medium, or hyaluronic acid. After incubation, the cells adherent to the coated plates were harvested and the levels of 3H-thymidine were measured.

Results: Animals treated with N,O-carboxymethyl chitosan gel and solution showed significantly (P < .01) reduced severity and area of adhesion formation. Murine fibroblasts and macrophages did not adhere to N,O-carboxymethyl chitosan-coated tissue culture plates, even in the presence of serum.

Conclusion: The application of N,O-carboxymethyl chitosan gel and solution significantly reduces the severity of postsurgical adhesion formation after cardiac surgery in the rabbit model. The inability of fibroblasts to adhere to N,O-carboxymethyl chitosan-coated surfaces suggests that N,O-carboxymethyl chitosan may act as a biophysical barrier.

Abbreviations and Acronyms dpm = disintegrations per minute; FCS = fetal calf serum; HA = hyaluronic acid; NOCC = N,O-carboxymethyl chitosan; sfRPMI = serum-free Roswell Park Memorial Institute [medium]




This article has been cited by other articles:


Home page
 J. Thorac. Cardiovasc. Surg.Home page
J. Zhou, J. M. Lee, P. Jiang, S. Henderson, and T. D. G. Lee
Reduction in postsurgical adhesion formation after cardiac surgery by application of N,O-carboxymethyl chitosan
J. Thorac. Cardiovasc. Surg., October 1, 2010; 140(4): 801 - 806.
[Abstract] [Full Text] [PDF]

Home page
 Ann. Thorac. Surg.Home page
J. B. Lopes, L. A. O. Dallan, L. F. P. Moreira, S. P. Campana Filho, P. S. Gutierrez, L. A. F. Lisboa, S. A. de Oliveira, and N. A. G. Stolf
Synergism Between Keratinocyte Growth Factor and Carboxymethyl Chitosan Reduces Pericardial Adhesions
Ann. Thorac. Surg., August 1, 2010; 90(2): 566 - 572.
[Abstract] [Full Text] [PDF]

Home page
 Interact CardioVasc Thorac SurgHome page
A. Bel, L. Kachatryan, P. Bruneval, S. Peyrard, C. Gagnieu, J.-N. Fabiani, and P. Menasche
A new absorbable collagen membrane to reduce adhesions in cardiac surgery
Interact CardioVasc Thorac Surg, February 1, 2010; 10(2): 213 - 216.
[Abstract] [Full Text] [PDF]

Home page
 Eur J Cardiothorac SurgHome page
J. B. Lopes, L. A. O. Dallan, S. P. Campana-Filho, L. A. F. Lisboa, P. S. Gutierrez, L. F. P. Moreira, S. A. Oliveira, and N. A. G. Stolf
Keratinocyte growth factor: a new mesothelial targeted therapy to reduce postoperative pericardial adhesions
Eur J Cardiothorac Surg, February 1, 2009; 35(2): 313 - 318.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ANN THORAC SURG ASIAN CARDIOVASC THORAC ANN EUR J CARDIOTHORAC SURG
J THORAC CARDIOVASC SURG ICVTS ALL CTSNet JOURNALS
Copyright © 2008 by The American Association for Thoracic Surgery.