HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Add to Personal Folders Download to citation manager Permission Requests Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Jones, M. T. Articles by Cooper, J. D. Search for Related Content PubMed PubMed Citation Articles by Jones, M. T. Articles by Cooper, J. D.

The Journal of Thoracic and Cardiovascular Surgery, Vol 96, 608-614, Copyright © 1988 by The American Association for Thoracic Surgery and The Western Thoracic Surgical Association

ARTICLES

A new model for assessment of lung preservation

MT Jones, C Hsieh, K Yoshikawa, GA Patterson and JD Cooper
Division of Thoracic Surgery, Toronto General Hospital, Ontario, Canada.

No completely satisfactory experimental model exists to compare different techniques of preservation currently used in the distant procurement of a lung allograft. A canine model of left lung transplantation is described in which an inflatable cuff is placed around each pulmonary artery. Each cuff is connected to a subcutaneous reservoir, which allows alternate occlusion of either pulmonary artery. Functional assessment of lung function is made during ventilation of both lungs and after a 10-minute period of perfusion to the native lung alone and then to the transplanted lung alone. Systemic and pulmonary artery pressures are recorded continuously, and measurement of arterial blood gases and oxygen uptake are made immediately after the operation and again at 3 days. The animal is then put to death and the lungs are excised and weighed. Five dogs underwent transplantation of the donor lung immediately after excision (mean ischemic time = 55 +/- 7 minutes). Similar values for oxygen tension and oxygen uptake were obtained postoperatively for the right lung (oxygen tension = 420 mm Hg, oxygen uptake = 101 ml/min) and the left lung (oxygen tension = 368 mm Hg, oxygen uptake = 108 ml/min). However, carbon dioxide tension was elevated (right lung = 41 mm Hg, left lung = 52 mm Hg). Mean pulmonary artery pressure increased during allograft perfusion (right lung = 14 mm Hg, left lung = 24 mm Hg), although systemic blood pressure was unchanged. Similar results were observed at 3 days. The mean weight of the native lung was 101 +/- 2 gm and that of the transplanted lung, 128 +/- 6 gm. This model achieves consistent survival and allows serial observations of the functional adequacy of an allograft compared with a normal contralateral lung.

This article has been cited by other articles:

				S.-P. Sommer, B. Gohrbandt, S. Fischer, J. M. Hohlfeld, G. Warnecke, M. Avsar, and M. Struber Glutathione improves the function of porcine pulmonary grafts stored for twenty-four hours in low-potassium dextran solution J. Thorac. Cardiovasc. Surg., September 1, 2005; 130(3): 864 - 869. [Abstract] [Full Text] [PDF]

				S. Fischer, D. Hopkinson, M. Liu, and S. Keshavjee RAFFINOSE IMPROVES THE FUNCTION OF RAT PULMONARY GRAFTS STORED FOR TWENTY-FOUR HOURS IN LOW-POTASSIUM DEXTRAN SOLUTION J. Thorac. Cardiovasc. Surg., March 1, 2000; 119(3): 488 - 492. [Abstract] [Full Text] [PDF]

				E. A. Bacha, P. Herve, S. Murakami, A. Chapelier, G.-M. Mazmanian, V. de Montpreville, H. Detruit, J.-M. Libert, P. Dartevelle, and P.-S. U. L. T. Group LASTING BENEFICIAL EFFECT OF SHORT-TERM INHALED NITRIC OXIDE ON GRAFT FUNCTION AFTER LUNG TRANSPLANTATION J. Thorac. Cardiovasc. Surg., September 1, 1996; 112(3): 590 - 598. [Abstract] [Full Text]

				H. Ichinari, T. Shimizu, M. Yoshioka, Y. Matsuzaki, K. Shibata, and Y. Koga EFFECTS OF PORTAL VENOUS INOCULATION WITH DONOR SPLENOCYTES ON LUNG ALLOGRAFT SURVIVAL IN DOGS J. Thorac. Cardiovasc. Surg., August 1, 1996; 112(2): 300 - 305. [Abstract] [Full Text]

				M. Aoe, K. Okabayashi, J. D. Cooper, and G. A. Patterson HYPERINFLATION OF CANINE LUNG ALLOGRAFTS DURING STORAGE INCREASES REPERFUSION PULMONARY EDEMA J. Thorac. Cardiovasc. Surg., July 1, 1996; 112(1): 94 - 102. [Abstract] [Full Text]

				D. A. Fullerton, R. C. McIntyre Jr., M. B. Mitchell, D. N. Campell, and F. L. Grover Lung transplantation with cardiopulmonary bypass exaggerates pulmonary vasomotor dysfunction in the transplanted lung J. Thorac. Cardiovasc. Surg., February 1, 1995; 109(2): 212 - 217. [Abstract] [Full Text]

				S. Sundaresan, O. Lima, H. Date, A. Matsumura, H. Tsuji, H. Obo, M. Aoe, T. Mizuta, and J. D. Cooper Lung preservation with low-potassium dextran flush in a primate bilateral transplant model Ann. Thorac. Surg., November 1, 1993; 56(5): 1129 - 1135. [Abstract] [PDF]

				A. J. B. Kirk, I. W. Colquhoun, and J. H. Dark Lung preservation: A review of current practice and future directions Ann. Thorac. Surg., October 1, 1993; 56(4): 990 - 1000. [Abstract] [PDF]

				R. J. Novick, K. R. Reid, L. Denning, J. Duplan, A. H. Menkis, and F. Neil McKenzie Prolonged preservation of canine lung allografts: The role of prostaglandins Ann. Thorac. Surg., May 1, 1991; 51(5): 853 - 859. [Abstract] [PDF]