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The Journal of Thoracic and Cardiovascular Surgery, Vol 116, 744-751, Copyright © 1998 by The American Association for Thoracic Surgery and The Western Thoracic Surgical Association
NOTE: Fulltext is available only in pdf format
J Dorfman, M Duong, A Zibaitis, MP Pelletier, D Shum-Tim, C Li and RC Chiu
OBJECTIVE: Implanting myoblasts derived from autologous skeletal muscle,
that is, satellite cells, for myocardial replacement has many advantages
when compared with implanting either fetal cardiac myocytes (ethical and
donor availability issues) or established cell lines (oncogenicity).
Furthermore, autologous myoblasts do not require immunosuppression. The
feasibility of satellite cell differentiation into muscle fibers, after
implantation into the myocardium, was confirmed by means of a unique
cell-labeling technique. METHODS: Myoblasts (satellite cells) isolated from
the skeletal muscle of adult rats are labeled with
4',6-diamidino-2-phenylindone, which binds to DNA and to the protein
tubulin to form a fluorescent complex, and implanted into the left
ventricular wall of isogenic rats. The specimens are harvested 1 to 4 weeks
after myoblast implantation. Histologic sections are examined under a
fluorescent microscope. RESULTS: The labeling efficiency of satellite cells
with 4',6-diamidino-2-phenylindole is nearly 100%. In 4 specimens, the
progressive differentiation of implanted myoblasts into fully developed
striated muscle fibers can be observed. CONCLUSION: Our earlier studies of
autologous myoblast implantation into the cryoinjured myocardium of dogs
suggested that these cells could differentiate into cardiac myocytes.
However, it had been difficult to firmly establish these findings with the
use of cell markers, thereby proving that the neomyocardium had indeed been
derived from the implanted myoblasts. In this study, using
4',6-diamidino-2- phenylindole as a satellite cell marker, we were able to
demonstrate that the implanted satellite cells did in fact differentiate
into fully developed, labeled muscle fibers. Because of the obvious
advantages of using autologous donor myoblasts, the clinical application of
this approach may provide a novel strategy for the future management of
heart failure.
ARTICLES
Myocardial tissue engineering with autologous myoblast implantation
Division of Cardiothoracic Surgery, McGill University, Montreal, Quebec, Canada.
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