| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
J Thorac Cardiovasc Surg 2003;125:1217-1228
© 2003 The American Association for Thoracic Surgery
Cardiothoracic Transplantation |
From the Department of Cardiovascular Surgerya and the Institute of Pathological Anatomy,b Albert-Ludwigs-University Medical Center, Freiburg, Germany.
Supported by the Clinical Research Center II of the Albert-Ludwigs-University Freiburg, grant No B4. HOE 642 was a gift of Hoechst AG, Frankfurt/Main, Germany. Leukocyte filters were a gift of Pall GmbH, Dreieich, Germany.
Received for publication May 29, 2002. Revisions requested July 30, 2002; revisions received Aug 7, 2002. Accepted for publication Aug 15, 2002. Address for reprints: Juergen Martin, MD, Department of Cardiovascular Surgery, Albert-Ludwigs-University, Hugstetter Str 55, D-79106 Freiburg, Germany (E-mail: Martin{at}ch11.ukl.uni-freiburg.de).
 |
Abstract |
|---|
 Top Abstract IntroductionMaterials and methodsResultsDiscussionAppendix: DiscussionReferences |
|---|
|
Introduction |
|---|
|
TopAbstractIntroductionMaterials and methodsResultsDiscussionAppendix: DiscussionReferences |
|---|
|
1 The use of marginal donors could help to expand the donor pool. Furthermore, hearts with prolonged ischemia times (>4 hours) and hearts from non-heart-beating donors (NHBDs) could be a source of additional donor organs. At present, these hearts are usually rejected in consideration of an increased risk of primary graft failure.
Previous studies in the pig model by our group have shown that successful heart transplantation from NHBDs after 30 minutes of normothermic ischemia is possible if controlled reperfusion with blood cardioplegia is performed along with some new reperfusion modalities. 2,3 These modalities include leukocyte depletion, supplementation with the sodium-hydrogen exchange inhibitor HOE 642, and start of reperfusion with tepid instead of normothermic blood cardioplegia. Despite encouraging results in these short-term experiments, cardiac output of the hearts was severely impaired, and irreversible myocardial damage could not be excluded. Therefore further experiments with longer observation times seemed to be necessary.
The purpose of this study was to evaluate the performance of hearts harvested from NHBDs 24 hours after transplantation in comparison with control hearts. The registration of pressure-volume loops by using the conductance catheter method provided a detailed analysis of myocardial contractility. In addition, measurements of regional myocardial perfusion, metabolic parameters (eg, oxygen consumption), and quantification of myocardial damage by means of histologic examinations enabled complex assessment of these hearts.
Further refinements of the myocardial protection strategy were introduced to minimize ischemia-reperfusion damage. First, blood cardioplegia was supplemented with adenosine. Adenosine has potent cardioprotective properties involving metabolic changes, inhibition of neutrophils, and relaxation of vasculature. 4 Second, controlled reperfusions with blood cardioplegia were performed after completion of each anastomosis to reduce ischemic damage during implantation of the donor heart.
|
Materials and methods |
|---|
|
TopAbstractIntroductionMaterials and methodsResultsDiscussionAppendix: DiscussionReferences |
|---|
Surgical procedure
After median sternotomy, 500 IE/kg heparin was given to all pigs. A 7F polyurethane catheter was inserted into the left atrium through the left atrial appendage to monitor left atrial pressure. A 12-gauge cannula was placed into the ascending aorta of the donor hearts for application of cardioplegia. This cannula was also used for exsanguination of the animals. After procurement, the donor hearts were stored in ice-cold cardioplegic solution.
Cardiopulmonary bypass
The heart-lung machine (Stöckert, Munich, Germany) was filled with a priming of 1000 mL of hydroxyethyl starch. We used a pediatric oxygenator (D705; Dideco, Mirandola, Italy), systemic heater-cooler (Jostra, Hirrlingen, Germany), and arterial filter (D733, Dideco). Cannulation of the ascending aorta was performed by using a 16F aortic cannula. Cannulation of the superior and inferior venae cavae was achieved through the right atrium with 20F and 28F cannulas. A 9F vent catheter was inserted into the left atrium to avoid distention of the left ventricle. The blood flow was 2.2 to 2.5 L/min.
Orthotopic heart transplantation
Transplantation was performed in a biatrial technique. The left azygos vein, a special feature of the porcine anatomy, was dissected in the donor. Left azygos vein and the coronary sinus of the recipient remained intact. A small catheter was placed in the coronary sinus of the donor heart to obtain blood samples for metabolic investigations.
At the end of the experiment, the animals were killed by means of an intravenous injection of potassium chloride.
Control group (n = 6)
Hearts were harvested in a clinically comparable manner. Cardiac arrest was induced by 1500 mL of Bretschneider's HTK solution (Custodiol; Dr Franz Köhler, Chemie GmbH, Alsbach-Hähnlein, Germany; 4°C; perfusion pressure 40-50 mm Hg) applied through the aortic root. The hearts were stored for 3 hours in ice-cooled HTK solution and transplanted orthotopically. No reperfusion modifications were used in this group.
NHBD group (n = 6)
Circulatory arrest was induced by means of exsanguination. After zero blood pressure, the animals were left undisturbed for 30 minutes. The hearts were beating empty, heart frequency decreased continuously, and complete cardiac arrest was observed 8 to 12 minutes after zero blood pressure.
Thereafter, controlled reperfusion with 1000 mL of blood cardioplegia (blood cardioplegic solution for controlled reperfusion; Dr Franz Köhler, Chemie GmbH, Germany) was performed through the aortic root (temperature of 10°C-15°C, perfusion pressure of 40 mm Hg). Blood cardioplegia was leukocyte depleted (Pall BC1; Pall GmbH, Dreieich, Germany) and supplemented with 379 mg/L HOE 642 (Hoechst AG, Frankfurt, Germany) and 100 mg/L adenosine (Sigma-Aldrich). Hearts were excised, stored in ice-cold blood cardioplegic solution for 3 hours, and transplanted orthotopically. During implantation, controlled reperfusion with 300 mL of leukocyte-depleted blood cardioplegia (20°C at 40 mm Hg) through the aortic root was performed after completion of each anastomosis. Blood cardioplegia was supplemented with HOE 642 and adenosine. After completion of the last anastomosis, controlled reperfusion was continued with leukocyte-depleted blood for 20 minutes. The temperature of reperfusate was 25°C initially and was stepwise increased to 37°C after 20 minutes before the aortic clamp was released. After the start of reperfusion, adenosine at 140 µg x kg-1 x min-1 was administered intravenously for 60 minutes.
In both groups weaning from cardiopulmonary bypass was attempted 60 minutes after the start of reperfusion. For inotropic support, 0.1 µg x kg-1 x min-1 epinephrine was administered.
Hemodynamic measurements
Cardiac output was measured by using the thermodilution technique.
Pressure-volume loops were registered by using the conductance catheter technique (Leycom CFL 512; CD Leycom, Zoetermeer, The Netherlands). A 7F pigtail conductance catheter (electrode spacing of 8 mm; Sentron, Roden, The Netherlands) and a 3F pressure-tip catheter (Millar, Houston, Tex) were introduced through apical incisions of the left and right ventricle. Hemodynamic parameters (dp/dt, end-systolic elastance, and preload recruitable stroke work [PRSW]) were calculated by using Leycom CFL software.
Regional myocardial blood flow
Regional myocardial blood flow (RMBF) was measured with fluorescent microspheres. Through a polyurethane cannula, 1.5 mL (1.5 x 106) of fluorescent polystyrene microspheres (diameter of 15 µm; fluorescent labels: orange, blue-green, and red; Molecular Probes, Eugene, Ore) mixed with 8.5 mL of heparinized fresh blood was injected into the left atrium. The injection was performed over a period of 60 seconds.
A reference blood sample was taken from the ascending aorta. Aspiration of the reference sample (withdrawal rate, 7.5 mL/min) was started 15 seconds before application of the microspheres and was continued for 45 seconds after completion of the injection.
Tissue and blood digestion
From each heart, midventricular slices were obtained from the left (n = 4) and right (n = 4) ventricular wall and from the septum (n = 4). The ventricular samples were divided into a subendocardial and subepicardial specimen. Each of the 20 samples was weighed (Precision Weigher; Sartorius GmbH, Göttingen, Germany). The mean wet weight of the tissue samples was 0.9 ± 0.2 g. Processing of FMS was performed by using the so-called sedimentation technique. 5,6
Measurement of fluorescence
Fluorescence was determined with a luminescence spectrophotometer (Perkin Elmer 650-10LC, Überlingen, Germany) with an excitation wavelength of 427 to 570 nm and an emission wavelength of 468 to 598 nm.
Calculation of myocardial blood flow
The fluorescent signal in the solution is directly proportional to the number of microspheres present in the sample. Flow to each tissue piece was calculated by using the following formula:
(1)
Qx = (Flux/Fluref) x Qref.,
where Qx is defined as blood flow in the tissue sample (in milliliters per gram times minutes), Qref is defined as the withdrawal rate of the reference blood sample (in milliliters per minute), Flux is defined as fluorescence intensity in the tissue sample (in units per milliliter), and Fluref is defined as fluorescence intensity in the reference blood sample (in units per milliliter).
Metabolic parameters
Blood samples were obtained from the arterial blood, blood cardioplegia, and coronary sinus to measure blood gases and glucose and lactate concentrations (ABL System 625/AS 117; Radiometer Medical, Copenhagen, Denmark).
Myocardial oxygen consumption was calculated by using the following equation:
(2)
MVO2 = (A-V) x MBF,
where MVO2 is defined as myocardial oxygen consumption (in milliliters per 100 grams per minute), A is defined as arterial oxygen concentration (milliliters of oxygen per milliliter of blood), V is defined as coronary sinus oxygen concentration (in milliliters of oxygen per milliliter of blood), and MBF is defined as myocardial blood flow (in milliliters per 100 grams per minute).
For calculation of oxygen consumption at baseline and 4 and 24 hours after transplantation, total myocardial blood flow was derived from regional myocardial blood flow, according to the ratio of left ventricular/right ventricular/septal heart weight (0.65/0.25/0.1). This ratio was determined by means of postmortem examinations of the hearts. Of course, this method provides only an approximate calculation of total myocardial blood flow.
Extraction of oxygen, glucose, and lactate was calculated by using the following equation:
(3)
where E is defined as the extraction quotient (in percentage), A is defined as the arterial substrate concentration, and V is defined as the coronary sinus substrate concentration.
Myocardial specific enzymes
The routine test for assessment of creatine kinase MB (CK-MB) does not produce reliable data in pigs. 3 Therefore we used an agarose electrophoresis test (REP-CK/LD-isoenzyme combo method; Helena Laboratories, Beaumont, Tex). This test analyses the percentage of CK-MB in relation to the total CK. Total CK was measured photometrically with an enzymatic test (CK [NAC] AU 5000 Analyser System; Merck, Darmstadt, Germany).
Histologic examinations
Specimens of the left midventricular wall were embedded in paraffin and stained with hematoxylin-eosin and Luxol fast blue. Luxol fast blue staining provides a safe identification of irreversibly damaged myocardium. Therefore the ratio of damaged myocytes in relation to normal myocytes was assessed in 10 high-power fields at 40-fold magnification by 2 blinded observers.
Statistical analysis
Statistical analysis was performed with a statistical computer program (Prism; Graph Pad Software, San Diego, Calif). Group statistics were expressed as means ± SD.
Two-group comparisons of the posttransplant values with the baseline values, subendocardial versus subepicardial RMBF, and left ventricle versus right ventricle were made by running a paired t test with a 2-tailed P value. For analysis of repeated measures of one variable, a repeated measures analysis of variance was used. Intergroup comparisons were performed by using an unpaired t test.
|
Results |
|---|
|
TopAbstractIntroductionMaterials and methodsResultsDiscussionAppendix: DiscussionReferences |
|---|
Time for weaning
All animals could be weaned from cardiopulmonary bypass after 58 ± 28 minutes in the control group and 74 ± 18 minutes in the NHBD group (not significant).
Hemodynamics
Data on henodynamics are shown in Tables 1 and 2. Hemodynamics remained stable throughout the observation period of 24 hours. For inotropic support, 0.1 µg x kg-1 x min-1 of epinephrine was given.
|
|
. In both groups cardiac output and ejection fraction of both ventricles after transplantation were significantly impaired compared with baseline values. Differences between the groups after transplantation were not significant. Heart rate, left atrial and central venous pressure, and systemic vascular and pulmonary vascular resistance were significantly increased after transplantation.
The dp/dtmax and dp/dtmin, left ventricular PRSW, and left ventricular end-systolic elastance (Ees) were not significantly changed 24 hours after transplantation compared with the baseline values (Table 2). In contrast, right ventricular PRSW and Ees were significantly increased in both groups.
Regional myocardial blood flow
RMBF data are shown in Figure 1. Measurements at baseline revealed homogeneous distribution of blood flow without significant differences between the left ventricle, right ventricle, and septum (Figure 1, A). After transplantation, RMBF was significantly increased in both ventricles but not in the septum (Figure 1, B). Myocardial blood flow in the subendocardial layer was higher compared with that in the subepicardial layer in both ventricles (Figure 1, C and D). The subendothelial/subepicardial myocardial blood flow ratio did not change significantly over the observation period. Intergroup differences in RMBF were not significant.
|
, A). After 24 hours, oxygen consumption had completely normalized. Intergroup differences were not significant. In the NHBD group oxygen extraction was significantly decreased compared with that seen in the control group 5, 20, 30, and 60 minutes after the start of reperfusion. Thereafter, intergroup differences were not significant (Figure 2, B).
|
|
|
|
). In both groups systemic glucose levels remained increased over 24 hours. At baseline, no arteriovenous difference of blood glucose levels could be measured. Within the first 10 minutes after the start of reperfusion, arteriovenous glucose extraction reached negative values in the NHBD group but not in the control group. After 24 hours, glucose extraction was 3.4% in the control group and 2.4% in the experimental group. Intergroup differences were significant 1 and 5 minutes after the start of reperfusion.
Systemic lactate levels were significantly higher in the experimental group within the first 60 minutes after the start of reperfusion (Figure 4). In the control group myocardial lactate production could be observed within the first 20 minutes after aortic unclamping. In contrast, the hearts of the NHBD group were able to consume lactate. Intergroup differences were significant during the first 10 minutes only.
Metabolic activity during controlled reperfusions of the donor heart in the NHBD group is shown in Figure 5. Starting the controlled reperfusion in the donor after 30 minutes of normothermic ischemia at a myocardial temperature of 28°C, oxygen extraction reached greater than 70%, indicating good metabolic activity. At the end of this first reperfusion, myocardial temperature had decreased to 13°C before the heart was excised and stored in ice-cold solution.
Reperfusion in the recipient was started after completion of the first anastomosis at a temperature of 15°C. Temperature was stepwise increased to 20°C. Oxygen extraction ranged between 65% and 75%.
Myocardial specific CK-MB
Myocardial specific CK-MD data are given in Figure 6. CK-MB was significantly increased 30 minutes to 24 hours after transplantation in both groups. The CK-MB level reached a peak after 2 hours and decreased 24 hours after heart transplantation. Differences between the control and NHBD groups were not significant.
|
|
|
|
Discussion |
|---|
|
TopAbstractIntroductionMaterials and methodsResultsDiscussionAppendix: DiscussionReferences |
|---|
Hemodynamics
Cardiac output and ejection fraction after transplantation were significantly decreased compared with baseline values in both groups (Table 1). This was associated with a dramatic increase in pulmonary vascular resistance, a common problem after cardiopulmonary bypass in pigs. 7 PRSW, Ees, dp/dtmax, and dp/dtmin were not diminished after heart transplantation, indicating normal contractile function (Table 2).
In contrast, right ventricular, but not left ventricular, PRSW and Ees were significantly increased. A possible explanation of this phenomenon could be methodical problems related to the enormously increased afterload after transplantation. Interpretation of pressure-volume loops provides reliable assessment of contractility independent of preload conditions, but results can be significantly influenced by changing afterload conditions. 8 An increase in right ventricular PRSW index and Ees might result from the enhanced afterload after transplantation and might not reflect an increase in myocardial contractility.
Regional myocardial blood flow
Coronary blood flow was increased 4 and 24 hours after transplantation in both groups (Figure 1). This enhancement might be due to the application of epinephrine and has been observed by other investigators. 9,10 In contrast, ischemic myocardial damage has been shown to be associated with increased coronary resistance and restricted coronary flow reserve. 11,12 The finding of increased RMBF in the present study shows no evidence of severe myocardial damage.
Metabolic activity
Myocardial oxygen consumption of porcine myocardium at rest (12 mL x kg-1 x min-1) is comparable with that of human hearts and reflects aerobic metabolism. Measurements 4 and 24 hours after transplantation revealed no significant changes in both groups (Figure 2). After 30 minutes of normothermic ischemia, myocardium was able to extract 75% of oxygen from blood cardioplegia during the first controlled reperfusion in the donor. Similar results could be obtained after the start of reperfusion during implantation of the donor heart (Figure 5).
Our data indicate full viability and preserved myocardial function after transplantation of these ischemically compromised hearts. Successful transplantation was possible by using a new preservation strategy based on the concept of integrated myocardial protection. 13 Myocardial preservation strategies must address a broad spectrum of pathophysiologic aspects. In addition to the established principles of controlled reperfusion, further refinements have been used to prevent leukocyte-mediated damage and preserve endothelial function.
Leukocyte depletion
Leukocyte filtration of blood cardioplegia has been shown to reduce reperfusion damage in several experimental and clinical studies. 14-16 Leukocyte filters can remove more than 90% of blood leukocytes, but our data show that they become less functional after blood delivery of greater than 1000 mL and have to be exchanged (Figure 7).
Adenosine
Damaged hearts exhibit loss of endothelium-dependant factors and reduced nitric oxide formation. Impairment of endothelial function plays an important role in ischemia-reperfusion injury and has been underestimated in previous preservation strategies. 17 We must consider endothelial stunning and develop efforts to reduce this injury during myocardial protection. 18
Adenosine has been shown to have broad-spectrum cardioprotective effects by acting through multiple mechanisms on neutrophils, endothelium, and myocytes. 4,19
Berne 20 described the key role of adenosine in the autoregulation of coronary blood flow. Furthermore, because of stimulation of myocardial A1 receptors, adenosine activates adenosine triphosphate-sensitive potassium channels and attenuates myocardial stunning. 21 Applied as an adjunct to standard cold blood cardioplegia in hearts exposed to 30 minutes of normothermic ischemia, adenosine reversed the postischemic systolic dysfunction. 22
Intermittent reperfusion during donor heart implantation
Another attempt to minimize myocardial damage in heart transplantation is the application of blood cardioplegia during implantation of the donor heart. We started antegrade reperfusion with tepid blood cardioplegia (15°C) after completion of the first anastomosis to avoid the high temperature gradient between the reperfusate and the myocardium. Blood cardioplegia was substrate enriched and supplemented with high doses of HOE 642 and adenosine. Various modifications of early reperfusion with blood cardioplegia have been described. 23-25 Clinical investigations have shown that intermittent cold blood cardioplegia during implantation is superior to the conventional technique with respect to the incidence of spontaneous defibrillation, return to sinus rhythm, time of mechanical ventilation, and need for inotropic support. 23,24
Clinical implications
The critical shortage of transplantable organs necessitates the use of unconventional donors. Cautious liberalization of donor criteria is justified. The use of NHBDs has been proposed as one way to increase the donor pool for kidneys and livers worldwide. 26-29 Routine transplantation of islets and lungs from NHBDs can be expected within the near future. 30,31 Isolated instances of clinical heart transplantation from NHBDs have been reported. 32,33 The results are encouraging and have increased the awareness of these types of donors for organ procurement. The use of marginal donors should be cautiously pursued as one way to help alleviate the current shortage of donor organs.
As more experience in the use of NHBD is gathered, this should serve as an impetus to further expand our knowledge of biologic or physiologic alteration in these donors, which can be minimized by newer technology. Refinements of the preservation strategy are necessary to provide successful transplantation of hearts from marginal donors or NHBDs. Traditional myocardial protection techniques are sufficient for good donor hearts and short ischemic times. Liberalization of donor criteria requires broader use of controlled reperfusion, leukocyte depletion, and other protective additives in heart transplantation.
Limitations of the study
Increased afterload after heart transplantation, as indicated by pulmonary and systemic vascular resistance, might influence contractility parameters (eg, cardiac output and PRSW). Therefore comparison between hemodynamic baseline data and posttransplant data bears problems. In contrast, comparison between the NHBD group and the control group is appropriate. Pharmacologic interventions to decrease pulmonary vascular resistance would have been helpful to avoid these problems and to adopt the model more to the clinical setting.
NHBD hearts were obtained after arresting the hearts by means of exsanguination. In the face of a potential clinical setting with NHBDs (so-called controlled NHBDs), it has to be considered that donors are taken off the ventilator. There is some evidence that death from asphyxia causes more myocardial damage than death from exsanguination. 34 Further experiments are necessary with respect to the detrimental effects of anoxia and higher filling pressures to evaluate the efficiency of our myocardial preservation strategy.
Moreover, the detrimental effects of brain death are not considered in this experimental model. Possibly, brain death of the donor could further impair the function of the transplanted hearts.
In conclusion, transplantation of ischemically compromised hearts harvested after 30 minutes of normothermic ischemia is possible in this experimental model without relevant irreversible damage. Contractility, myocardial perfusion, metabolic activity, and histologic damage are comparable with those of hearts harvested from beating-heart donors.
|
Appendix: Discussion |
|---|
|
TopAbstractIntroductionMaterials and methodsResultsDiscussionAppendix: DiscussionReferences |
|---|
Dr Martin. Yes, that is a good question. We built on experiments we had performed some years ago in an acute model with an observation time of 2 hours, and there we performed some pilot studies. We published the results in the European Journal of Cardio-Thoracic Surgery. We found that by using only crystalloid cardioplegia, we were not able to wean the hearts from the extracorporeal circulation; this was only possible with blood cardioplegia. We have introduced, compared with these further experiments, some additional refinements, and these refinements include the use of adenosine, which has been investigated by many investigators in the field of myocardial protection. Adenosine and HOE 642 have a lot of properties to improve myocardial protection, and we have given them as an adjunct to the intermittent reperfusions of the transplanted hearts during implantation. After each anastomosis, we gave 300 mL of blood cardioplegia.
Dr Henry M. Spotnitz (New York, NY). Can you comment on whether preservation of the control group was optimal?
Dr Martin. In the control group we used hearts from beating-heart donors, and they were transplanted in a standard fashion. The aorta was clamped, the Bretschneider's cardioplegia was infused, and the hearts were excised, stored for 4 hours in Bretschneider's solution, and transplanted. No reperfusion modifications were performed. The aortic clamp was released after implantation.
Dr Spotnitz. But you did not resuscitate them with the same protocol you used for the still hearts?
Dr Martin. No, we did not do it because our intention was to compare these new preservation strategies in NHBDs with the procedures in hearts transplanted in a clinically standard procedure.
Dr Spotnitz. How would you compare the enzyme release that you observed in the control group with clinical results?
Dr Martin. We found, in our clinical observations, that CK-MB is increased after nearly each heart transplantation. I think you cannot avoid an increase in troponin or CK-MB.
Dr Spotnitz. To a comparable degree?
Dr Martin. Yes. The problem is that we have to use other enzyme tests than those we use in the clinical arena because the CK-MB of the pig does not react well with the clinical tests, and therefore the values are not completely comparable.
Dr Gerald D. Buckberg (Los Angeles, Calif). It is a lovely study showing the beauty of dealing with reperfusion injury in hearts that have been dead for basically 30 minutes and transplanted. Can you comment on the role of the HOE 642 and calcium metabolism and leukocytes to make us understand what mechanisms you think are important in your treatment?
Dr Martin. Thank you for this question, Professor Buckberg. HOE 642 is a sodium-proton exchange inhibitor, and it has been tested in several in vivo and in vitro models. The principle is that the sodium-proton exchange is increased during ischemia and reperfusion because of the increased concentration of protons within the cell. The sodium-proton exchange inhibitor is activated to transport these protons to the extracellular space, and therefore the sodium influx is increased. The consequence of an increased sodium influx is that this sodium will be transported outside of the cell by use of the sodium-calcium exchange.
Normally, you have the sodium-potassium exchange, but this enzyme is adenosine triphosphate dependent, and it will not work after 1 minute of normothermic ischemia. Because of the lack of the sodium-potassium exchange, the cell has to use the sodium-calcium exchange, and the last consequence of this mechanism is progressive calcium overload of the cell. Calcium deposits in the mitochondria are responsible for the irreversible myocardial damage. By blocking the sodium-proton exchange in the beginning, you have more acidosis of the cell, but this is not so deleterious as a calcium overload.
Dr Sidney Levitsky (Boston, Mass). If that is your hypothesis for using the sodium-proton exchange inhibitor, which is a valid hypothesis, shouldn't you have given the drug before the ischemic period, when the drug has its greatest effect, rather than after the fact during the reperfusion period?
Dr Martin. Thank you, Dr Levitsky. That is a very good question. Of course, several studies have shown that the sodium-proton exchange inhibitor has the strongest effect if it is given before ischemia, but we initially tried to perform this transplantation without special donor pretreatment. This was in accordance to the ethical considerations, especially in Germany and Europe, that it is forbidden to treat a potential donor or organ donor only for the purpose of organ procurement. Now things have changed a bit, and I think that in the next experiments we should give the sodium-proton exchange inhibitor before the procurement of the donor heart.
Dr Paul Grundeman (Utrecht, The Netherlands). In this model you exsanguinated the animals so the hearts were anoxic in a fashion that the ventricles actually were not under tension; there was no wall tension. Now, if you would, let's say, fibrillate the animal and you would then take the heart, what would it look like? I am just asking this question because you might think of the following clinical situation: you have a person found dead, and 30 minutes after you found this person dead, you want to take the heart. So this is a different situation. This is sort of artificial.
Dr Martin. Okay, I agree with you, it is of importance which method to use to induce cardiac arrest. We have chosen this method because we wanted to use the blood of the donor for the preparation of blood cardioplegia, but in fact, in the clinical arena the most common procedure is the controlled NHBDs. These patients are disconnected from the respirator, and they die as a result of asphyxia. There are some investigations by other authors that have shown that asphyxia is more deleterious for the myocardium than exsanguination or other techniques. I think this is a major limitation of our study that we have not investigated the influence of asphyxia on the performance of the hearts, and we should do this in further investigations.
|
Acknowledgments |
|---|
|
Footnotes |
|---|
|
References |
|---|
|
TopAbstractIntroductionMaterials and methodsResultsDiscussionAppendix: DiscussionReferences |
|---|
This article has been cited by other articles:
|
|
 |
|
  F. Beyersdorf The use of controlled reperfusion strategies in cardiac surgery to minimize ischaemia/reperfusion damage Cardiovasc Res, July 15, 2009; 83(2): 262 - 268. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. J. Collins, S. L. Moainie, B. P. Griffith, and R. S. Poston Preserving and evaluating hearts with ex vivo machine perfusion: an avenue to improve early graft performance and expand the donor pool. Eur. J. Cardiothorac. Surg., August 1, 2008; 34(2): 318 - 325. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Kotani, K. Ishino, S. Osaki, O. Honjo, T. Suezawa, K. Kanki, C. Yutani, and S. Sano Efficacy of MCI-186, a free-radical scavenger and antioxidant, for resuscitation of nonbeating donor hearts J. Thorac. Cardiovasc. Surg., June 1, 2007; 133(6): 1626 - 1632. [Abstract] [Full Text] [PDF]
|
|
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| ANN THORAC SURG | ASIAN CARDIOVASC THORAC ANN | EUR J CARDIOTHORAC SURG |
| J THORAC CARDIOVASC SURG | ICVTS | ALL CTSNet JOURNALS |
P < .05, NHBD group versus control group. Measurements were performed before procurement of the donor heart (baseline) and after the start of reperfusion in the recipient (control group, aortic unclamping; NHBD group, hot shot after completion of the last anastomosis). There was decreased oxygen extraction in the experimental group during the first 60 minutes after the start of reperfusion. There were no significant intergroup differences between 1 and 24 hours after heart transplantation.
